AB100562

Human IL-1 beta ELISA Kit

Name: Human IL-1 beta ELISA Kit (ab100562) | Abcam
Brand: Abcam Limited
SKU: AB100562
Price: 780 USD
Availability: InStock

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(52 Publications)

Human IL-1 beta ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 beta in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

3 Images

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB100562)

sELISA

Unknown

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB100562)

Representative standard curve using ab100562

sELISA

Lab

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB100562)

Hu IL-1 beta measured in biological fluids showing quantity (pg) per mL of tested sample (U937 : stimulated 24h with 10ng/mL PMA and 6h with LPS 1microgram/mL -- THP-1 : stimulated 24h with 10ng/mL PMA)

sELISA

Unknown

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB100562)

Representative standard curve using ab100562

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.3 pg/mL

Range

0.48 - 100 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 beta in plasma and cell culture supernatants. (Human IL-1 beta concentration is quite low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for human IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous human IL-1 beta in normal serum with ab100562, only in serum spiked with human IL-1 beta.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 110 %", "average": "= 100.43" }, { "sample": "Plasma", "range": "90 - 107 %", "average": "= 99.66" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100562-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100562-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100562-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 wells)", "number":"AB100562-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100562-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-1 beta Standards (lyophilized)", "number":"AB100562-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-1 beta", "number":"AB100562-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100562-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100562-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100562-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).

See full target information IL1B

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Publications (52)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0295837 PubMed38335214

2024

Poly-(ADP-ribose) polymerases inhibition by olaparib attenuates activities of the NLRP3 inflammasome and of NF-κB in THP-1 monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Khamis Mustafa,Ying Han,Dan He,Ying Wang,Nan Niu,Pedro A Jose,Yinong Jiang,Jeffrey B Kopp,Hewang Lee,Peng Qu

Journal of cell science 136: PubMed37051862

2023

Inflammatory macrophage to hepatocyte signals can be prevented by extracellular vesicle reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Ghosh,Kyo Sasaki,Isabel Aranzazu Pulido Ruiz,Kayla E King,Steven A Weinman,Ann L Wozniak

Oncology letters 25:225 PubMed37153045

2023

Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Junmei Li,Feng Yu,Bo Wang,Bing Liu

Translational neurodegeneration 12:10 PubMed36895045

2023

Inflammasome activation under high cholesterol load triggers a protective microglial phenotype while promoting neuronal pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina de Dios,Xenia Abadin,Vicente Roca-Agujetas,Marina Jimenez-Martinez,Albert Morales,Ramon Trullas,Montserrat Mari,Anna Colell

European journal of neurology 30:3440-3450 PubMed36263629

2022

Intestinal histomorphological and molecular alterations in patients with Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Bellini,Laura Benvenuti,Chiara Ippolito,Daniela Frosini,Cristina Segnani,Francesco Rettura,Andrea Pancetti,Lorenzo Bertani,Vanessa D'Antongiovanni,Giovanni Palermo,Eleonora Del Prete,Luca Antonioli,Vincenzo Nardini,Riccardo Morganti,Carolina Pellegrini,Nunzia Bernardini,Roberto Ceravolo,Matteo Fornai,Massimo Bellini

Experimental and therapeutic medicine 24:603 PubMed35949322

2022

ROCK2 knockdown alleviates LPS-induced inflammatory injury and apoptosis of renal tubular epithelial cells via the NF-κB/NLRP3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinfeng Qian,Linjun Yang

Microbiology and immunology 66:433-441 PubMed35568971

2022

Diagnostic value of lncRNA NORAD in pulmonary tuberculosis and its regulatory role in Mycobacterium tuberculosis infection of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Wenna Sun,Xiong He,Xiushuang Zhang,Xiaomeng Wang,Wen Lin,Xiaofeng Wang,Yan Liang

Allergologia et immunopathologia 50:115-123 PubMed35789410

2022

Sevoflurane ameliorates LPS-induced inflammatory injury of HK-2 cells through Sirtuin1/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Peipei,Wang Ping,Yin Miaomiao,Wang Shuo

Regenerative therapy 20:95-106 PubMed35509266

2022

Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Niu,Jing Xu,Yujuan Yan

Journal of healthcare engineering 2022:1473597 PubMed35035808

2022

Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Liu,Chunjing Li,Qiuyang Wang,Haiyan Hu,Chunhong Li,Jiuguang Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-1 beta ELISA Kit

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB100562)

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB100562)

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB100562)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (52)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Product promise