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AB108865

Human IL-1 beta ELISA Kit

5

(1 Review)

|

(4 Publications)

Human IL-1 beta ELISA Kit is a Sandwich ELISA for the measurement of Human IL-1 beta in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

1 Images
Sandwich ELISA - Human IL-1 beta ELISA Kit (AB108865)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB108865)

Representative Standard Curve using ab108865.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

>= 0.8 pg/mL

Range

3.91 - 250 pg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-1 beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta concentrations in cell culture supernatants, serum and plasma.

An IL-1 beta specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-1 beta specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-1 beta captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108865-1x96Tests", "assetComponentDetails": [ { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108865-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB108865-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108865-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108865-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108865-CMP04", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108865-CMP05", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108865-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human IL-1 beta Antibody", "number":"AB108865-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 well strips)", "number":"AB108865-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IL-1 beta Standard", "number":"AB108865-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).
See full target information IL1B
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of dental sciences 18:636-644 PubMed37021271

2023

Myeloid-derived growth factor regulates high glucose-mediated apoptosis of gingival fibroblasts and induce AKT pathway activation and nuclear factor κB pathway inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Gao,Zhenqiang Li,Wenxiao Chang,Yanyan Liu,Nan Zhang

Journal of inflammation (London, England) 19:17 PubMed36266722

2022

Disulfiram alleviates acute lung injury and related intestinal mucosal barrier impairment by targeting GSDMD-dependent pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiping Zhao,Hong Wang,Jintao Zhang,Fuwei Ou,Junfei Wang,Tian Liu,Jinxiang Wu

Diabetology & metabolic syndrome 13:122 PubMed34715911

2021

Dysregulation of miR-638 in diabetic nephropathy and its role in inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Lin,Dan Song,Suo Zhang,Ping Li

Molecular neurobiology 56:3576-3590 PubMed30151726

2018

Cocaine Mediated Neuroinflammation: Role of Dysregulated Autophagy in Pericytes.

Applications

Unspecified application

Species

Unspecified reactive species

Susmita Sil,Fang Niu,Eric Tom,Ke Liao,Palsamy Periyasamy,Shilpa Buch
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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