Human IL-1 beta ELISA kit is a high-sensitivity SimpleStep ELISA® assay designed for the quantification of human interleukin-1 beta (IL-1β) in with a sensitivity of 5.64 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric Sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Cited in over 90 publications
Images
Publications
Filter by
- International journal of molecular sciences 24:2023Prdx6 Regulates Nlrp3 Inflammasome Activation-Driven Inflammatory Response in Lens Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBhavana Chhunchha,Rakesh Kumar,Eri Kubo,Priyanka Thakur,Dhirendra P SinghPubMed 38003466
- Experimental and therapeutic medicine 26:5752023IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia by alleviating endoplasmic reticulum stress and the inflammatory response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhou,Qigang Dai,Ning Su,Zhihui Liu,Jinxing HuPubMed 38023363
- Heliyon 9:e207712023Electroacupuncture combined with extracorporeal shock wave therapy improves pain symptoms and inflammatory factor levels in knee osteoarthritis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengfu Liu,Qiudan Chen,Qinggang Zhang,Kun Tao,Changhong Li,Baolei Chang,Weifeng Wang,Zhong WuPubMed 37842584
- Molecular medicine reports 28:2023Kynurenic acid inhibits macrophage pyroptosis by suppressing ROS production via activation of the NRF2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuwei Gao,Xiaohui Guo,Yunpeng Zhou,Jie Du,Chengbo Lu,Lei Zhang,Siyuan Sun,Shengfang Wang,Yang LiPubMed 37772394
- Cellular & molecular biology letters 28:662023MiR-15b-5p and PCSK9 inhibition reduces lipopolysaccharide-induced endothelial dysfunction by targeting SIRT4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Martino,Nunzia D'Onofrio,Anna Balestrieri,Luigi Mele,Celestino Sardu,Raffaele Marfella,Giuseppe Campanile,Maria Luisa BalestrieriPubMed 37587410
- Frontiers in microbiology 14:12360122023Differential expression analysis of miRNAs in macrophage-derived exosomes in the tuberculosis-infected bone microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhicheng Sun,Xiaoyang Pang,Xiyang Wang,Hao ZengPubMed 37601387
- Immunity, inflammation and disease 11:e9052023Circ_0035292 knockdown alleviates lipopolysaccharide (LPS)-induced WI-38 cell apoptosis and inflammatory injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Guo,Zhouzhen Li,Chen ChengPubMed 37382271
- iScience 26:1071582023HDAC3 promotes macrophage pyroptosis via regulating histone deacetylation in acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Li,Bohao Liu,Ruyuan He,Guorui Li,Rui Xiong,Tinglv Fu,Donghang Li,Chenzhen Xu,Bo Wang,Qing GengPubMed 37404376
- Acta biochimica Polonica 70:371-3772023Endoplasmic reticulum stress involves the high glucose-induced nucleus pulposus cell pyroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaochun Xiong,Ying Wu,Hengguo Long,Tianzi Liang,Wenqing Liang,Xiaogang Huang,Guijin LiPubMed 37307502
- Experimental and therapeutic medicine 25:2762023ZBTB16 eases lipopolysaccharide‑elicited inflammation, apoptosis and degradation of extracellular matrix in chondrocytes during osteoarthritis by suppressing GRK2 transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Xiong,Lihua Chen,Yue Huang,Guanyu Lu,Cai Chen,Jiao Nong,Haida PanPubMed 37206562
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14.06 - 900 pg/mL
- Assay time
- 2h 30m
- Sensitivity
- = 5.64 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
1 product for Alternative Version
Target data
Function
Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).
Alternative names
IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin
What's included?
Recommended products
Human IL-1 beta ELISA kit is a high-sensitivity SimpleStep ELISA® assay designed for the quantification of human interleukin-1 beta (IL-1β) in with a sensitivity of 5.64 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric Sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Cited in over 90 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14.06 - 900 pg/mL
- Assay time
- 2h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5.64 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 8 | C.V. 4.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 98 | Range 96 - 100 % |
Sample type Serum | Average % = 103 | Range 101 - 105 % |
Sample type EDTA Plasma | Average % = 93 | Range 90 - 96 % |
Sample type Heparin Plasma | Average % = 100 | Range 99 - 101 % |
Sample type Citrate plasma | Average % = 86 | Range 84 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-1 beta ELISA Kit ab214025 is a rapid single-wash 90-min ELISA kit to measure Human ALT in cell culture supernatant, serum, plasma. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.
How the assay works
Human ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only. Tissue extract samples have not been tested with this kit. This kit is incompatible with plasma- heparin and cell extract samples.
Human IL-1 beta ELISA Kit ab214025 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IL-1 beta ELISA Kit ab214025
Human IL-1 beta ELISA Kit ab214025 has been used to study inflammation in various physio-pathological contexts such as pneumonia(1), osteoarthritis(2), and diabetes(3).
References:
(1)Jinxing Hu et al. 2023, PMID: 38023363,(2)Zhong Wu et al. 2023, PMID: 37842584,
(3)Raffaele Marfella, et al. 2023, PMID: 37005586
Save your precious samples
Our 384-well Human IL-1 beta ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Monkey IL-1beta Antibody Pair - BSA and Azide free Human/Monkey IL-1beta Antibody Pair - BSA and Azide free ab241807
- Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) ab242561
- Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector) Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector) ab242986
Related and recommended products
Human IL-1 beta ELISA Kit ab214025 is commonly used to measure IL-1 beta as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human IL-6 ELISA kit Human IL-6 ELISA Kit ab178013
- Human TNF alpha ELISA kit Human TNF alpha ELISA Kit ab181421
- Human IL-18 ELISA kit Human IL-18 ELISA Kit ab215539
To measure IFN gamma at a lower dynamic range (12.5-1600 pg/mL):
- Human IFN gamma High Sensitivity ELISA kit Human IFN gamma High Sensitivity ELISA Kit ab236895
Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Example of human IL-1beta standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Example of human IL-1beta standard curve in Sample Diluent NS. The IL-1beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Interpolated concentrations of native IL-1beta in human PHA stimulated PBMC supernatant and LPS stimulated THP-1 supernatant samples.
The concentrations of IL-1beta were measured in duplicates, interpolated from the IL-1beta standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50% and 100% THP-1 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1301 pg/mL in PBMC supernatant and 734 pg/mL in THP-1 supernatant.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated values are plotted (mean +/- SD, n=2). IL-1beta was measured in 2 donor serum samples (30 pg/mL and 140 pg/mL) and the remaining 8 samples measured less than the lowest point of the IL-1beta standard curve.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.
Conditioned media was harvested after 48 hours. IL-1beta was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-1beta were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1273 pg/mL in PHA stimulated PBMC supernatant. There was no detectable signal in unstimulated supernatant.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
THP-1 cells were cultured unstimulated or stimulated with 5 µg/mL Lipopolysaccharide (LPS).
Conditioned media was harvested after 48 hours. IL-1beta was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-1beta were measured in duplicate and interpolated from the IL-1beta standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 718 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (Human IL-1 beta ELISA Kit, Fluorescent ab229384).
This data was collected using Human IL-1 beta ELISA Kit, Fluorescent ab229384, which uses the same recombinant antibody pair and standard protein. Human IL-1 beta concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-1 beta ELISA kit (Human IL-1 beta ELISA Kit, Fluorescent ab229384). Wild-type and IL-1 beta knockout THP-1 cells (Human IL1B knockout THP-1 cell line ab273762) were assessed in duplicate (n=2). Cells were either treated with LPS (100 ng/ml, 3 h) then ATP (5 mM, 45 min) to induce expression of IL-1 beta or not treated. Data are represented as the mean and error bars represent standard deviation.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-1beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)
Linearity of dilution.
Recombinant IL-1beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com