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The Human IL-1 beta ELISA kit is a high-sensitivity SimpleStep ELISA® assay designed for the quantification of human interleukin-1 beta (IL-1β) in with a sensitivity of 5.64 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive human IL-1 beta sandwich ELISA Kit:
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Pre-coated microplate – Includes 12 x 8-well strips, allowing flexible assay setup
- Cited in over 90 publications


Images

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

14.06 - 900 pg/mL

Assay time

2h 30m

Sensitivity

= 5.64 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).

Alternative names

What's included?

10 x 96 Tests
Components
10X Human IL-1beta Capture Antibody
10 x 600 µL
10X Human IL-1beta Detector Antibody
10 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 200 mL
Antibody Diluent 4BI
10 x 6 mL
Human IL-1beta Lyophilized Recombinant Protein (ab9617)
2 x 10 Vial
Plate Seals
10 x 1 Unit
Sample Diluent NS (ab193972)
2 x 250 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
10 x 1 Unit
Stop Solution
1 x 120 mL
TMB Development Solution
1 x 120 mL

Recommended products

The Human IL-1 beta ELISA kit is a high-sensitivity SimpleStep ELISA® assay designed for the quantification of human interleukin-1 beta (IL-1β) in with a sensitivity of 5.64 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

Easy-to-use, rapid, sensitive human IL-1 beta sandwich ELISA Kit:
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Pre-coated microplate – Includes 12 x 8-well strips, allowing flexible assay setup
- Cited in over 90 publications

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

14.06 - 900 pg/mL

Assay time

2h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 5.64 pg/mL

Precision

Intra assay

Sample

Overall

n
8
mean
-
SD
-
C.V.

4.8

Inter assay

Sample

Overall

n
3
mean
-
SD
-
C.V.

5.6

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 98

Range

96 - 100 %

Sample type

Serum

Average %

= 103

Range

101 - 105 %

Sample type

EDTA Plasma

Average %

= 93

Range

90 - 96 %

Sample type

Heparin Plasma

Average %

= 100

Range

99 - 101 %

Sample type

Citrate plasma

Average %

= 86

Range

84 - 88 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human IL-1 beta ELISA Kit ab214025 is a rapid single-wash 90-min ELISA kit to measure Human IL-1 beta in cell culture supernatant, serum and plasma . This SimpleStep ELISA® sensitivity is 5.64 pg/mL.

How the assay works

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay protocol summary

Human IL-1 beta ELISA Kit ab214025 protocol summary:

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hour at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Alternative assay kits

Need to design your own immunoassay? We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Human/Monkey IL-1beta Antibody Pair - BSA and Azide free Human/Monkey IL-1beta Antibody Pair - BSA and Azide free ab241807,
- Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) Anti-IL-1 beta antibody [EPR18815-27] - BSA and Azide free (Capture) ab242561,
- Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector) Anti-IL-1 beta antibody [EPR18815-128] - BSA and Azide free (Detector) ab242986

Getting the best performance

ASSAY SPECIFICITY

This kit recognizes both native and recombinant human IL-1beta protein in serum, plasma, and cell culture supernatant samples only.
Cell and tissue extract samples have not been tested with this kit.

CROSS REACTIVITY

Recombinant mouse IL-1beta and human IL-1 receptor antagonist were prepared at 50 ng/mL and 225 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.

INTERFERENCE

Recombinant human Interleukin-1 receptor type 1 was prepared at 50 ng/mL and 225 pg/mL and tested for interference. No interference with was observed.

SPECIES REACTIVITY

This kit recognizes human IL-1beta protein.
Other species reactivity was determined by measuring serum samples of various species, interpolating the IL-1beta protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the IL-1beta protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
Other species reactivity not determined.

Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

How other researchers are using this kit

Human IL-1 beta ELISA Kit ab214025 has been used to study inflammation in various physio-pathological contexts such as pneumonia (1), osteoarthritis (2), and diabetes (3).

References: 1-Jinxing Hu et al. 2023, PMID: 38023363, 2-Zhong Wu et al. 2023, PMID: 37842584, 3-Raffaele Marfella, et al. 2023, PMID: 37005586

Related and recommended products

Human IL-1 beta ELISA Kit ab214025 is commonly used to measure IL-1 beta as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human IL-6 ELISA kit Human IL-6 ELISA Kit ab178013
- Human TNF alpha ELISA kit Human TNF alpha ELISA Kit ab181421
- Human IL-18 ELISA kit Human IL-18 ELISA Kit ab215539

To measure IFN gamma at a lower dynamic range (12.5-1600 pg/mL):

- Human IFN gamma High Sensitivity ELISA kit Human IFN gamma High Sensitivity ELISA Kit ab236895

Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Example of human IL-1beta standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Example of human IL-1beta standard curve in Sample Diluent NS. The IL-1beta standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Interpolated concentrations of native IL-1beta in human PHA stimulated PBMC supernatant and LPS stimulated THP-1 supernatant samples.

    The concentrations of IL-1beta were measured in duplicates, interpolated from the IL-1beta standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50% and 100% THP-1 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1301 pg/mL in PBMC supernatant and 734 pg/mL in THP-1 supernatant.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated values are plotted (mean +/- SD, n=2). IL-1beta was measured in 2 donor serum samples (30 pg/mL and 140 pg/mL) and the remaining 8 samples measured less than the lowest point of the IL-1beta standard curve.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

    Conditioned media was harvested after 48 hours. IL-1beta was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-1beta were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1273 pg/mL in PHA stimulated PBMC supernatant. There was no detectable signal in unstimulated supernatant.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    THP-1 cells were cultured unstimulated or stimulated with 5 µg/mL Lipopolysaccharide (LPS).

    Conditioned media was harvested after 48 hours. IL-1beta was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-1beta were measured in duplicate and interpolated from the IL-1beta standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 718 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (Human IL-1 beta ELISA Kit, Fluorescent ab229384).

    This data was collected using Human IL-1 beta ELISA Kit, Fluorescent ab229384, which uses the same recombinant antibody pair and standard protein. Human IL-1 beta concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-1 beta ELISA kit (Human IL-1 beta ELISA Kit, Fluorescent ab229384). Wild-type and IL-1 beta knockout THP-1 cells (Human IL1B knockout THP-1 cell line ab273762) were assessed in duplicate (n=2). Cells were either treated with LPS (100 ng/ml, 3 h) then ATP (5 mM, 45 min) to induce expression of IL-1 beta or not treated. Data are represented as the mean and error bars represent standard deviation.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native IL-1beta was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    Linearity of dilution.

    Recombinant IL-1beta was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit (ab214025)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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