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AB46052

Human IL-1 beta ELISA Kit

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(83 Publications)

Human IL-1 beta ELISA Kit is a Sandwich ELISA for the measurement of Human IL-1 beta in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

3 Images
ELISA - Human IL-1 beta ELISA Kit (AB46052)
  • ELISA

Lab

ELISA - Human IL-1 beta ELISA Kit (AB46052)

Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).

ELISA - Human IL-1 beta ELISA Kit (AB46052)
  • ELISA

Lab

ELISA - Human IL-1 beta ELISA Kit (AB46052)

IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and 1 ug x mL-1 LPS (Sigma) for the last 6 hours (P+L).

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB46052)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB46052)

Representative Standard Curve using ab46052

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.5 pg/mL

Range

15.6 - 500 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.

A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.

This kit will recognize both endogenous and recombinant Human IL-1 beta.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 6", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Intra", "sample": "Sample 6", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 105 %", "average": "= 89" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46052-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46052-CMP04" }, { "size":"4 x 1 Vial", "name":"IL1 beta standard", "number":"AB46052-CMP11" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46052-CMP09" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46052-CMP02" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46052-CMP07" }, { "size":"2 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 well strips)", "number":"AB46052-CMP10" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46052-CMP05" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46052-CMP03" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46052-CMP12" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-1 beta", "number":"AB46052-CMP01" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46052-CMP06" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46052-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46052-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46052-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL1 beta standard", "number":"AB46052-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46052-CMP09", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46052-CMP05", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46052-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-1 beta", "number":"AB46052-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46052-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 beta Microplate (12 x 8 well strips)", "number":"AB46052-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46052-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46052-CMP12", "productcode":"" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46052-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46052-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).
See full target information IL1B
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Publications (83)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular diabetology 24:203 PubMed40361188

2025

Diabetes mellitus aggravates myocardial inflammation and oxidative stress in aortic stenosis: a mechanistic link to HFpEF features.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa Herwig,Marcel Sieme,Andrea Kovács,Muchtiar Khan,Andreas Mügge,Wolfgang E Schmidt,Ferhat Elci,Shan Sasidharan,Peter Haldenwang,Jan Wintrich,Benjamin Sasko,Ibrahim Akin,Máthé Domokos,Francesco Paneni,Ibrahim El-Battrawy,Zoltán V Varga,Francisca Saraiva,Adelino F Leite-Moreira,Péter Ferdinandy,Loek van Heerebeek,Inês Falcão-Pires,Nazha Hamdani

Journal of clinical medicine 14: PubMed40364209

2025

Assessment of Serum Cytokine Levels in Keratoconus Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Noor Alqudah,Nosayba Al-Azzam,Leen El Taani,Abdallah Sharayah,Mohammad Al Qudah,Khawlah Mhedat,Suha Tahat

Medicine 104:e41699 PubMed40068086

2025

Effect of adjuvant cisternostomy on the prognosis of patients with severe traumatic brain injury: A single center's retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Chen,Wenxin Zheng,Wei Wang,Daokun Shu,Dongdong Xu,Qiquan Zhang,Bin Wang,Yi Zhuo,Raorao Yuan,Shiqi Cheng

Drug development research 86:e70065 PubMed39950329

2025

Exosomal ALKBH5 Alleviates Vascular Calcification by Suppressing Cell Apoptosis via m6A-Modified GSDME.

Applications

Unspecified application

Species

Unspecified reactive species

Guian Xu,Qingman Li,Lijie Zhu,Tingjie Yang,Yapan Yang,Honghui Yang

Methods in molecular biology (Clifton, N.J.) 2878:273-291 PubMed39546268

2024

Methodologies for Mitochondrial Omic Profiling During Spaceflight.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph W Guarnieri,JangKeun Kim,Douglas C Wallace,Christopher E Mason,Masafumi Muratani,Afshin Beheshti

Cells 13: PubMed39451241

2024

Activation of Mammary Epithelial and Stromal Fibroblasts upon Exposure to Metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Jamilah H Alshehri,Huda K Al-Nasrallah,Mysoon M Al-Ansari,Abdelilah Aboussekhra

Folia neuropathologica 62:156-170 PubMed39165203

2024

Inhibition of AIM2 expression enhance treatment effect of osimertinib in treatment of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Wang,Hongzhuang Zhang,Qianqian Wei

Pediatric pulmonology 59:3371-3382 PubMed39115441

2024

Eclipta prostrata improves alveolar development of bronchopulmonary dysplasia via suppressing the NLRP3 inflammasome in a DLD-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Zheng,Zhen Tan,Danying Zhu,Dongying Zhao,Chengbo Liu,Shunchun Wang,Xingyun Wang,Yongjun Zhang

Psychiatry and clinical neurosciences 78:473-481 PubMed38923201

2024

Cholesterol profiling reveals 7β-hydroxycholesterol as a pathologically relevant peripheral biomarker of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Junghee Ha,Go Eun Kwon,Yumi Son,Soo Ah Jang,So Yeon Cho,Soo Jin Park,Hyunjeong Kim,Jimin Lee,Juseok Lee,Dongryul Seo,Myeongjee Lee,Do Yup Lee,Man Ho Choi,Eosu Kim

Non-coding RNA research 9:294-306 PubMed38505310

2024

DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Zhenyu Yang,Xiuming He,Yeyang Wang,Dixin Luo,Wangyang Xu,Hongtao Zhang,Xiaozhong Zhou
View all publications
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Product promise

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