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AB325901

Human IL-1 beta ELISA Kit, Chemiluminescent

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Human IL-1 beta ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IL-1 beta with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 5.49 - 4000 pg/ml with a sensitivity of 3.95 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

6 Images
Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)
  • sELISA

Lab

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)
  • sELISA

Lab

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)

Standard curve comparison between the Chemiluminescent Human IL-1 beta SimpleStep ELISA and colorimetric Human IL-1 beta SimpleStep ELISA (ab214025) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)

THP-1 cells were cultured unstimulated or stimulated with 5 µg/mL Lipopolysaccharide (LPS). Conditioned media was harvested after 48 hours. IL-1 beta was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-1 beta were measured in duplicate and interpolated from the IL-1 beta standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 718 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)

Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours. IL-1 beta was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-1 beta were measured in duplicate, interpolated from the IL-1 beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 1,273 pg/mL in PHA stimulated PBMC supernatant. There was no detectable signal in unstimulated supernatant.

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)

Serum from ten individual healthy human male donors was measured in duplicate. Interpolated values are plotted (mean +/- SD, n=2). IL-1 beta was measured in 2 donor serum samples (30 pg/mL and 140 pg/mL) and the remaining 8 samples measured less than the lowest point of the IL-1 beta standard curve.

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Kit, Chemiluminescent (AB325901)

Interpolated concentrations of native IL-1 beta in human PHA stimulated PBMC supernatant and LPS stimulated THP-1 supernatant samples. The concentrations of IL-1 beta were measured in duplicates, interpolated from the IL-1 beta standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 50% and 100% THP-1 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1 beta concentration was determined to be 1,301 pg/mL in PBMC supernatant and 734 pg/mL in THP-1 supernatant.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.95 pg/mL

Range

5.49 - 4000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human IL-1 beta ELISA Kit, Chemiluminescent (ab325901) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-1 beta in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Supernatant with a sensitivity of 3.95 pg/mL.

How the assay works

Human IL-1 beta ELISA Kit, Chemiluminescent (ab325901) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-1 beta (ab325901) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-1 beta antibody [EPR18815-27] BSA and Azide free (Capture) ab242561
- Anti-IL-1 beta antibody [EPR18815-128] BSA and Azide free (Detector) ab242986
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.8" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" } ]
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Recovery

[ { "sample": "Serum", "range": "101 - 105 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "84 - 88 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "99 - 101 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "90 - 96 %", "average": "= 93" }, { "sample": "Cell culture supernatant", "range": "96 - 100 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).
See full target information IL1B
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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