Human IL-1 beta ELISA Kit, Fluorescent is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-1 beta , Fluorescent in Human in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Allergologia et immunopathologia 50:176-1862022Dimethyl itaconate inhibits LPS-induced inflammatory release and apoptosis in alveolar type II epithelial and bronchial epithelial cells by activating pulmonary surfactant proteins A and D.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Shi,Huai-Qing YinPubMed 36335462
- Immunity, inflammation and disease 10:e6922022HDAC4 depletion ameliorates IL-13-triggered inflammatory response and mucus production in nasal epithelial cells via activation of SIRT1/NF-κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHangyu Xu,Lingjun Wang,Huaqun Chen,Hefei CaiPubMed 36301023
- Cardiovascular therapeutics 2022:52993702022Colchicine Does Not Reduce Abdominal Aortic Aneurysm Growth in a Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Phie,Shivshankar Thanigaimani,Pacific Huynh,Raghuveeran Anbalagan,Corey S Moran,Robert Kinobe,Joseph V Moxon,Matt A Field,Smriti M Krishna,Jonathan GolledgePubMed 36262119
- Journal of molecular medicine (Berlin, Germany) 100:963-9712022Leukocyte cytokine responses in adult patients with mitochondrial DNA defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKalpita R Karan,Caroline Trumpff,Marissa Cross,Kristin M Engelstad,Anna L Marsland,Peter J McGuire,Michio Hirano,Martin PicardPubMed 35635577
- Mediators of inflammation 2022:84476752022Vitronectin, a Novel Urinary Proteomic Biomarker, Promotes Cell Pyroptosis in Juvenile Systemic Lupus Erythematosus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Zhang,Wenxu Pan,Hongli Wang,Cheng Zhi,Yanhao Lin,Ping Wu,Qi Ren,Ping Wei,Rui Chen,Feng Li,Ying Xie,Chun Kwok Wong,Hong Tang,Zhe Cai,Wanfu Xu,Huasong ZengPubMed 35462789
- Oxidative medicine and cellular longevity 2022:96579332022Ferrostatin-1 and 3-Methyladenine Ameliorate Ferroptosis in OVA-Induced Asthma Model and in IL-13-Challenged BEAS-2B Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Yang,Yunxiao ShangPubMed 35154576
- Bioengineered 12:4995-50032021Inhibition of HMGB1/RAGE axis suppressed the lipopolysaccharide (LPS)-induced vicious transformation of cervical epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLifang You,Hongyin Cui,Fen Zhao,Huier Sun,Huanxin Zhong,Guoli Zhou,Xuejun ChenPubMed 34369271
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.5 - 3600 pg/mL
- Assay time
- 2h
- Sensitivity
- = 2.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).
Alternative names
IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin
What's included?
Recommended products
Human IL-1 beta ELISA Kit, Fluorescent is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-1 beta , Fluorescent in Human in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.5 - 3600 pg/mL
- Assay time
- 2h
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Media | n 8 | C.V. 4.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Media | n 3 | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 98 | Range 96 - 100 % |
Sample type Serum | Average % = 103 | Range 101 - 105 % |
Sample type EDTA Plasma | Average % = 93 | Range 90 - 96 % |
Sample type Heparin Plasma | Average % = 100 | Range 99 - 101 % |
Sample type Citrate plasma | Average % = 86 | Range 84 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
IL-1 beta in vitro CatchPoint® ELISA kit is designed for the quantitative measurement of IL-1 beta protein in human serum, plasma and cell culture supernatants.
This CatchPoint ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint® ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
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6 product images
Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)
Example of human IL-1 beta standard curve in Sample Diluent NS.
The IL-1 beta standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)
Interpolated concentrations of native IL-1beta in human PHA stimulated PBMC supernatant and LPS stimulated THP-1 supernatant samples.
The concentrations of IL-1beta were measured in duplicates, interpolated from the IL-1beta standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50% and 100% THP-1 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1301 pg/mL in PBMC supernatant and 734 pg/mL in THP-1 supernatant.
Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated values are plotted (mean +/- SD, n=2). IL-1beta was measured in 2 donor serum samples (30 pg/mL and 140 pg/mL) and the remaining 8 samples measured less than the lowest point of the IL-1beta standard curve.
Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)
Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.
Conditioned media was harvested after 48 hours. IL-1beta was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-1beta were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1273 pg/mL in PHA stimulated PBMC supernatant. There was no detectable signal in unstimulated supernatant.
Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)
THP-1 cells were cultured unstimulated or stimulated with 5 µg/mL Lipopolysaccharide (LPS).
Conditioned media was harvested after 48 hours. IL-1beta was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-1beta were measured in duplicate and interpolated from the IL-1beta standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 718 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.
Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)
Human IL-1 beta concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-1 beta ELISA kit (ab229384). Wild-type and IL-1 beta knockout THP-1 cells (Human IL1B knockout THP-1 cell line ab273762) were assessed in duplicate (n=2). Cells were either treated with LPS (100 ng/ml, 3 h) then ATP (5 mM, 45 min) to induce expression of IL-1 beta or not treated. Data are represented as the mean and error bars represent standard deviation.
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