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Human IL-1 beta ELISA Kit, Fluorescent is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-1 beta , Fluorescent in Human in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (AB229384), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (AB229384), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (AB229384), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (AB229384), expandable thumbnail
  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (AB229384), expandable thumbnail

Publications

Key facts

Detection method
Fluorescent
Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
3.5 - 3600 pg/mL
Assay time
2h
Sensitivity
= 2.6 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

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Target data

Function

Potent pro-inflammatory cytokine (PubMed:10653850, PubMed:12794819, PubMed:28331908, PubMed:3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744). Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed:28331908).

Alternative names

What's included?

1 x 96 Tests
Components
100X Stoplight Red Substrate
1 x 120 µL
10X Human IL-1beta Capture Antibody
1 x 600 µL
10X Human IL-1beta Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
500X Hydrogen Peroxide (H2O2, 3%)
1 x 50 µL
Antibody Diluent 4BI
1 x 6 mL
Human IL-1beta Lyophilized Recombinant Protein (ab9617)
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated Black 96-Well Microplate
1 x 1 Unit
Stoplight Red Substrate Buffer
1 x 12 mL

Recommended products

Human IL-1 beta ELISA Kit, Fluorescent is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-1 beta , Fluorescent in Human in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method
Fluorescent
Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
3.5 - 3600 pg/mL
Assay time
2h
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 2.6 pg/mL

Precision

Intra assay

Sample
Media
n
8
C.V.
4.8

Inter assay

Sample
Media
n
3
C.V.
5.6

Recovery

Sample specific recovery

Sample type
Cell culture supernatant
Average %
= 98
Range
96 - 100 %
Sample type
Serum
Average %
= 103
Range
101 - 105 %
Sample type
EDTA Plasma
Average %
= 93
Range
90 - 96 %
Sample type
Heparin Plasma
Average %
= 100
Range
99 - 101 %
Sample type
Citrate plasma
Average %
= 86
Range
84 - 88 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

IL-1 beta in vitro CatchPoint® ELISA kit is designed for the quantitative measurement of IL-1 beta protein in human serum, plasma and cell culture supernatants.

This CatchPoint ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint® ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Interleukin 1 beta (IL-1 beta) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

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6 product images

  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)

    Example of human IL-1 beta standard curve in Sample Diluent NS.

    The IL-1 beta standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)

    Interpolated concentrations of native IL-1beta in human PHA stimulated PBMC supernatant and LPS stimulated THP-1 supernatant samples.

    The concentrations of IL-1beta were measured in duplicates, interpolated from the IL-1beta standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50% and 100% THP-1 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1301 pg/mL in PBMC supernatant and 734 pg/mL in THP-1 supernatant.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)

    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated values are plotted (mean +/- SD, n=2). IL-1beta was measured in 2 donor serum samples (30 pg/mL and 140 pg/mL) and the remaining 8 samples measured less than the lowest point of the IL-1beta standard curve.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)

    Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

    Conditioned media was harvested after 48 hours. IL-1beta was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-1beta were measured in duplicate, interpolated from the IL-1beta standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 1273 pg/mL in PHA stimulated PBMC supernatant. There was no detectable signal in unstimulated supernatant.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)

    THP-1 cells were cultured unstimulated or stimulated with 5 µg/mL Lipopolysaccharide (LPS).

    Conditioned media was harvested after 48 hours. IL-1beta was measured in 100% unstimulated and LPS stimulated THP-1 supernatant. The concentrations of IL-1beta were measured in duplicate and interpolated from the IL-1beta standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-1beta concentration was determined to be 718 pg/mL in LPS stimulated THP-1 supernatant. There was no detectable signal in unstimulated supernatant.

  • Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384), expandable thumbnail

    Sandwich ELISA - Human IL-1 beta ELISA Kit, Fluorescent (ab229384)

    Human IL-1 beta concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-1 beta ELISA kit (ab229384). Wild-type and IL-1 beta knockout THP-1 cells (Human IL1B knockout THP-1 cell line ab273762) were assessed in duplicate (n=2). Cells were either treated with LPS (100 ng/ml, 3 h) then ATP (5 mM, 45 min) to induce expression of IL-1 beta or not treated. Data are represented as the mean and error bars represent standard deviation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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