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AB47351

Human IL-1 beta ELISA Set (without plates)

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Human IL-1 beta ELISA Set (without plates) is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 beta ELISA Set (without plates) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1F2, IL1B, Interleukin-1 beta, IL-1 beta, Catabolin

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Sandwich ELISA - Human IL-1 beta ELISA Set (without plates) (AB47351)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 beta ELISA Set (without plates) (AB47351)

IL-1 beta Standard curve ranging from 15.6 to 500 pg/ml

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

15.6 - 500 pg/mL

Assay time

3h 45m

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-1β Human in vitro ELISA Set is designed for the quantitative measurement of IL-1β in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

This ELISA Set is intended to measure human IL-1b in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

The IL-1b ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

Recognizes both natural and recombinant human IL-1b.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Potent pro-inflammatory cytokine (PubMed : 10653850, PubMed : 12794819, PubMed : 28331908, PubMed : 3920526). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed : 3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed : 12794819). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33377178, PubMed : 33883744). Acts as a sensor of S.pyogenes infection in skin : cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection (PubMed : 28331908).
See full target information IL1B
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