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AB100563

Human IL-1 R4 ELISA Kit (ST2)

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(2 Publications)

Human IL-1 R4 ELISA Kit (ST2) is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 R4 (ST2) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2

2 Images
Sandwich ELISA - Human IL-1 R4 ELISA Kit (ST2) (AB100563)
  • sELISA

Unknown

Sandwich ELISA - Human IL-1 R4 ELISA Kit (ST2) (AB100563)

Representative standard curve using ab100563

Sandwich ELISA - Human IL-1 R4 ELISA Kit (ST2) (AB100563)
  • sELISA

Unknown

Sandwich ELISA - Human IL-1 R4 ELISA Kit (ST2) (AB100563)

Representative standard curve using ab100563

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

1.65 - 1200 pg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-1 R4 ELISA Kit (ST2) (ab100563) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 R4 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human IL-1 R4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 R4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 R4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 105 %", "average": "= 98.43" }, { "sample": "Serum", "range": "98 - 117 %", "average": "= 109.6" }, { "sample": "Plasma", "range": "67 - 102 %", "average": "= 84.06" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100563-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100563-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100563-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100563-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-1 R4 Standard (lyophilized)", "number":"AB100563-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-1 R4", "number":"AB100563-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-1 R4 Microplate (12 x 8 wells)", "number":"AB100563-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100563-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100563-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100563-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
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Product protocols

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Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
See full target information IL1RL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS neglected tropical diseases 13:e0007974 PubMed31877138

2019

Dynamic changes of soluble ST2 levels predicted fatality and were involved in coagulopathy in dengue fever in the elderly.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Cheng Hsieh,Ming-Yuan Hong,Tzong-Shiann Ho,Ching-Chuan Liu,Guey-Chuen Perng,Chia-Chang Chuang

Inflammation 42:2072-2085 PubMed31429014

2019

Pleural Effusion IL-33/sST2 Levels and Effects of Low and High IL-33/sST2 Levels on Human Mesothelial Cell Adhesion and Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Ourania S Kotsiou,Rajesh M Jagirdar,Eleftherios D Papazoglou,Chrissi Hatzoglou,Konstantinos I Gourgoulianis,Sotirios G Zarogiannis
View all publications
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