JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213796

Human IL-1 RL1 ELISA Kit (ST2)

Be the first to review this product! Submit a review

|

(0 Publication)

Human IL-1 RL1 ELISA Kit (ST2) is a Sandwich ELISA for the measurement of Human IL-1 RL1 (ST2) in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

DER4, ST2, T1, IL1RL1, Interleukin-1 receptor-like 1, Protein ST2

1 Images
Sandwich ELISA - Human IL-1 RL1 ELISA Kit (ST2) (AB213796)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 RL1 ELISA Kit (ST2) (AB213796)

Human IL1RL1 ELISA Kit (ST2) (ab213796) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB223864

Human Von Willebrand Factor ELISA Kit

Recombinant|SimpleStep

ELISA - Human Von Willebrand Factor ELISA Kit (AB223864)

  • 0
  • 0
Assay Kits

AB240679

Human Elafin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Elafin ELISA Kit (AB240679)

  • 0
  • 0
Assay Kits

AB241383

Human Fibrinogen ELISA Kit - high sensitivity

Recombinant|SimpleStep

ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human IL-1 RL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ST2) (ab213796) is designed for the quantitative detection of Human IL1RL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-1 RL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: K19-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL1RL1amount of sample captured in plate.

Interleukin 1 receptor-like 1, also known as IL-1 RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by pro-inflammatory stimuli and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL-1 RL1), interleukin 1 receptor, type II (IL-1 R2) and interleukin 1 receptor like 2 (IL-1 RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "0.69 ng/mL", "standardDeviation": "0.06", "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "2.54 ng/mL", "standardDeviation": "0.157", "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "4.68 ng/mL", "standardDeviation": "0.229", "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "0.53 ng/mL", "standardDeviation": "0.028", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "2.05 ng/mL", "standardDeviation": "0.094", "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "4.31 ng/mL", "standardDeviation": "0.142", "coefficientOfVariability": "3.3" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213796-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213796-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human IL1RL1 antibody", "number":"AB213796-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL1RL1 coated Microplate (12 x 8 wells)", "number":"AB213796-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213796-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213796-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213796-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213796-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213796-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized recombinant Human IL1RL1 standard", "number":"AB213796-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213796-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213796-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.
Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.
style
left-column

Product protocols

style
left-column

Target data

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed : 35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed : 16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. Isoform B. Inhibits IL-33 signaling.
See full target information IL1RL1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com