Human IL-10 ELISA Kit (ab100549)

Human IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-10 in Human in Plasma, Cell culture supernatant, Serum samples.

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Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

Detection method: Colorimetric
Sample types: Plasma, Cell culture supernatant, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 2.34 - 150 pg/mL
Sensitivity: < 1 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Images

Target data

See full target information IL10

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

What's included?

1 x 96 Tests

Components

200X HRP-Streptavidin Concentrate

1 x 200 µL

20X Wash Buffer

1 x 25 mL

5X Assay Diluent B

1 x 15 mL

Assay Diluent A

1 x 30 mL

Biotinylated anti-Human IL-10

2 x 1 Vial

IL-10 Microplate (12 x 8 wells)

1 x 1 Unit

Recombinant Human IL-10 Standard (lyophilized)

2 x 1 Vial

Stop Solution

1 x 8 mL

TMB One-Step Substrate Reagent

1 x 12 mL

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Human IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-10 in Human in Plasma, Cell culture supernatant, Serum samples.

Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

Key facts

Detection method: Colorimetric
Sample types: Plasma, Cell culture supernatant, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 2.34 - 150 pg/mL
Assay Platform: Microplate
Sensitivity: < 1 pg/mL
General recovery: 100%

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Overall	n 0	mean -	SD -	C.V. < 10

Inter assay

Sample	n	mean	SD	C.V.
Sample Overall	n 0	mean -	SD -	C.V. < 12

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 98.37	Range 87 - 110 %
Sample type Serum	Average % = 101.94	Range 87 - 108 %
Sample type Plasma	Average % = 102.39	Range 93 - 109 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: -20°C
Appropriate long-term storage conditions: -20°C
Storage information: -20°C

Notes

Abcam's IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA^® range.

Produced using a non-baculovirus expression system.

Optimization may be required with urine samples

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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