AB100549

Human IL-10 ELISA Kit

Name: Human IL-10 ELISA Kit (ab100549) | Abcam
Brand: Abcam Limited
SKU: AB100549
Price: 780 USD
Availability: InStock

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(27 Publications)

Human IL-10 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-10 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

6 Images

Sandwich ELISA - Human IL-10 ELISA Kit (AB100549)

sELISA

Unknown

Sandwich ELISA - Human IL-10 ELISA Kit (AB100549)

Typical Standard Curve.

Representative standard curve using ab100549

ELISA - Human IL-10 ELISA Kit (AB100549)

ELISA

Lab

ELISA - Human IL-10 ELISA Kit (AB100549)

IL-10 measured in culture supernatants from control (-) or activated cell lines (dilution range 1/1-1/00), stimulated for 24 hours with 50 ng x mL^-1 of PMA (P)(ab120297) and with the addition of 1 ug x mL^-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants (dilution range 1/10-1/1000), from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).

ELISA

Lab

ELISA - Human IL-10 ELISA Kit (AB100549)

Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).

sELISA

Unknown

Sandwich ELISA - Human IL-10 ELISA Kit (AB100549)

Representative standard curve using ab100549

ELISA

Lab

ELISA - Human IL-10 ELISA Kit (AB100549)

Dilution curves (in assay diluent B) of human IL-10 (ab100549) and NIBSC standard (93/722). One ng of standard IL-10 corresponds to 0.94 (+/- 0.08) U NIBSC standard. Background signal subtracted (duplicates; +/- SD).

ELISA

Lab

ELISA - Human IL-10 ELISA Kit (AB100549)

Standard curve (assay diluent A) with background signal subtracted (duplicates; +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1 pg/mL

Range

2.34 - 150 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA^® range.

Produced using a non-baculovirus expression system.

Optimization may be required with urine samples

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 110 %", "average": "= 98.37" }, { "sample": "Serum", "range": "87 - 108 %", "average": "= 101.94" }, { "sample": "Plasma", "range": "93 - 109 %", "average": "= 102.39" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100549-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100549-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100549-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100549-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100549-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100549-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100549-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-10 Standard (lyophilized)", "number":"AB100549-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-10", "number":"AB100549-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 wells)", "number":"AB100549-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information IL10

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 19:80 PubMed39456006

2024

Repetitive transcranial magnetic stimulation alleviates motor impairment in Parkinson's disease: association with peripheral inflammatory regulatory T-cells and SYT6.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Xie,Bibiao Shen,Yuqi Luo,Hang Zhou,Zhenchao Xie,Shuzhen Zhu,Xiaobo Wei,Zihan Chang,Zhaohua Zhu,Changhai Ding,Kunlin Jin,Chengwu Yang,Lucia Batzu,K Ray Chaudhuri,Ling-Ling Chan,Eng-King Tan,Qing Wang

Journal of neuro-oncology 170:101-117 PubMed39143438

2024

Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM.

Applications

Unspecified application

Species

Unspecified reactive species

Songyun Zhao,Kaixiang Ni,Jiaheng Xie,Chao Cheng,Ning Zhao,Jinhui Liu,Wei Ji,Qi Wang,Pengpeng Zhang,Yuankun Liu

Immunity, inflammation and disease 12:e1146 PubMed38415977

2024

Knockdown of liver cancer cell-secreted exosomal PSMA5 controls macrophage polarization to restrain cancer progression by blocking JAK2/STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shujie Xie,Xiang Li,Jia Yan,Hua Yu,Shuhuai Chen,Kana Chen

Asian Pacific journal of cancer prevention : APJCP 24:3739-3748 PubMed38019231

2023

Regulation of NF-κB Expression by Thymoquinone; A Role in Regulating Pro-Inflammatory Cytokines and Programmed Cell Death in Hepatic Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Salah,Rasha Sleem,Amal Abd-Elaziz,Hany Khalil

Scientific reports 13:17344 PubMed37833265

2023

Increased blood immune regulatory cells in severe COVID-19 with autoantibodies to type I interferons.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Shirin Hafezi,Hawra Ali Hussain Alsayed,Balachandar Selvakumar,Mariam Wed Abdelaziz Eladham,Bushra Mdkhana,Ola Salam Bayram,Mohamad-Hani Temsah,Rabih Halwani

Experimental dermatology 33:e14926 PubMed37702410

2023

Vitamin D3 mitigates autoimmune inflammation caused by activation of myeloid dendritic cells in SLE.

Applications

Unspecified application

Species

Unspecified reactive species

Mingfang Li,Li Luo,Chuanchuan Lin,Bing Ni,Liyun Zou,Zhiqiang Song,Fei Hao,Yi Wu,Na Luo

Chemical biology & drug design 102:1202-1212 PubMed37599210

2023

Curcumin inhibits malignant behavior of colorectal cancer cells by regulating M2 polarization of tumor-associated macrophages and metastasis associated in colon cancer 1 (MACC1) expression.

Applications

Unspecified application

Species

Unspecified reactive species

Shuke Ge,Xu Sun,Limin Sang,Min Zhang,Xubo Yan,Qi Ju,Xuefeng Ma,Meng Xu

Frontiers in medicine 9:896409 PubMed36160124

2022

Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Xu,Juan Du,Ting-Ting Wei,Lin-Yi Chen,Xin-Xin Yang,Tu Bo,Han-Yu Liu,Ming-Zhu Xie,Tian-Shuo Zhao,Jun-Lian Yang,Fuqiang Cui,Wei-Wei Chen,Qing-Bin Lu

Chemotherapy 67:223-233 PubMed35649347

2022

Circular RNA hsa_circ_0068252 Functions in Cisplatin Resistance and Immune Response via miR-1304-5p/PD-L1 Axis in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Li,Jinhua Xu,Guofeng Wu,Yajun Ren,Xue Wang,Qianyun Zhang

International journal of molecular sciences 22: PubMed34576334

2021

Influence of Ce Substitution on Antimicrobial and Antibiofilm Properties of ZnCeFeO Nanoparticles (X = 0.0, 0.02, 0.04, 0.06, and 0.08) Conjugated with Ebselen and Its Role Subsidised with γ-Radiation in Mitigating Human TNBC and Colorectal Adenocarcinoma Proliferation In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed K Abdel-Rafei,Noura M Thabet,M I A Abdel Maksoud,M Abd Elkodous,Go Kawamura,Atsunori Matsuda,A H Ashour,Ahmed I El-Batal,Gharieb S El-Sayyad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-10 ELISA Kit

Sandwich ELISA - Human IL-10 ELISA Kit (AB100549)

ELISA - Human IL-10 ELISA Kit (AB100549)

ELISA - Human IL-10 ELISA Kit (AB100549)

Sandwich ELISA - Human IL-10 ELISA Kit (AB100549)

ELISA - Human IL-10 ELISA Kit (AB100549)

ELISA - Human IL-10 ELISA Kit (AB100549)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (27)

Product promise