Human IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-10 in Human in Plasma, Cell culture supernatant, Serum samples.
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- Asian Pacific journal of cancer prevention : APJCP 24:3739-37482023Regulation of NF-κB Expression by Thymoquinone; A Role in Regulating Pro-Inflammatory Cytokines and Programmed Cell Death in Hepatic Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Salah,Rasha Sleem,Amal Abd-Elaziz,Hany KhalilPubMed 38019231
- Scientific reports 13:173442023Increased blood immune regulatory cells in severe COVID-19 with autoantibodies to type I interferons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Shirin Hafezi,Hawra Ali Hussain Alsayed,Balachandar Selvakumar,Mariam Wed Abdelaziz Eladham,Bushra Mdkhana,Ola Salam Bayram,Mohamad-Hani Temsah,Rabih HalwaniPubMed 37833265
- Chemical biology & drug design 102:1202-12122023Curcumin inhibits malignant behavior of colorectal cancer cells by regulating M2 polarization of tumor-associated macrophages and metastasis associated in colon cancer 1 (MACC1) expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuke Ge,Xu Sun,Limin Sang,Min Zhang,Xubo Yan,Qi Ju,Xuefeng Ma,Meng XuPubMed 37599210
- Frontiers in medicine 9:8964092022Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Xu,Juan Du,Ting-Ting Wei,Lin-Yi Chen,Xin-Xin Yang,Tu Bo,Han-Yu Liu,Ming-Zhu Xie,Tian-Shuo Zhao,Jun-Lian Yang,Fuqiang Cui,Wei-Wei Chen,Qing-Bin LuPubMed 36160124
- Chemotherapy 67:223-2332022Circular RNA hsa_circ_0068252 Functions in Cisplatin Resistance and Immune Response via miR-1304-5p/PD-L1 Axis in Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Li,Jinhua Xu,Guofeng Wu,Yajun Ren,Xue Wang,Qianyun ZhangPubMed 35649347
- International journal of molecular sciences 22:2021Influence of Ce Substitution on Antimicrobial and Antibiofilm Properties of ZnCeFeO Nanoparticles (X = 0.0, 0.02, 0.04, 0.06, and 0.08) Conjugated with Ebselen and Its Role Subsidised with γ-Radiation in Mitigating Human TNBC and Colorectal Adenocarcinoma Proliferation In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed K Abdel-Rafei,Noura M Thabet,M I A Abdel Maksoud,M Abd Elkodous,Go Kawamura,Atsunori Matsuda,A H Ashour,Ahmed I El-Batal,Gharieb S El-SayyadPubMed 34576334
- Disease markers 2021:64232442021The Prognostic Determinant of Interleukin-10 in Patients with Acute Ischemic Stroke: An Analysis from the Perspective of Disease Management.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Sun,Shuhui Wang,Shanji NanPubMed 34336007
- Heliyon 7:e075512021Evaluation of circulating levels of Interleukin-10 and Interleukin-16 and dietary inflammatory index in Lebanese knee osteoarthritis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeina El-Ali,Germine El-Kassas,Fouad M Ziade,Nitin Shivappa,James R Hébert,Hassan Zmerly,Nisrine BissarPubMed 34337179
- Infection and immunity 89:2021Distinct Group B Sequence and Capsule Types Differentially Impact Macrophage Stress and Inflammatory Signaling Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca A Flaherty,David M Aronoff,Jennifer A Gaddy,Margaret G Petroff,Shannon D ManningPubMed 33558317
- Aging 13:11061-110822021microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the -dependent p38 MAPK axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhou,Suining Li,Liehong Yang,Daokang XiangPubMed 33744854
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.34 - 150 pg/mL
- Sensitivity
- < 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
Alternative names
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
What's included?
Recommended products
Human IL-10 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-10 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.34 - 150 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 1 pg/mL
- General recovery
- 100%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 98.37 | Range 87 - 110 % |
Sample type Serum | Average % = 101.94 | Range 87 - 108 % |
Sample type Plasma | Average % = 102.39 | Range 93 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human IL-10 ELISA Kit (Human IL-10 ELISA Kit (Interleukin-10) ab185986) from our SimpleStep ELISA® range.
Produced using a non-baculovirus expression system.
Optimization may be required with urine samples
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6 product images
ELISA - Human IL-10 ELISA Kit (ab100549)
IL-10 measured in culture supernatants from control (-) or activated cell lines (dilution range 1/1-1/00), stimulated for 24 hours with 50 ng x mL-1 of PMA (P)(Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants (dilution range 1/10-1/1000), from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).
ELISA - Human IL-10 ELISA Kit (ab100549)
Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).
ELISA - Human IL-10 ELISA Kit (ab100549)
Standard curve (assay diluent A) with background signal subtracted (duplicates; +/- SD).
ELISA - Human IL-10 ELISA Kit (ab100549)
Dilution curves (in assay diluent B) of human IL-10 (ab100549) and NIBSC standard (93/722). One ng of standard IL-10 corresponds to 0.94 (+/- 0.08) U NIBSC standard. Background signal subtracted (duplicates; +/- SD).
Sandwich ELISA - Human IL-10 ELISA Kit (ab100549)
Typical Stardard Curve.
Representative standard curve using ab100549
Sandwich ELISA - Human IL-10 ELISA Kit (ab100549)
Representative standard curve using ab100549
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