AB46034

Human IL-10 ELISA Kit

Name: Human IL-10 ELISA Kit (ab46034) | Abcam
Brand: Abcam Limited
SKU: AB46034
Price: 780 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(35 Publications)

Human IL-10 ELISA Kit is a sandwich ELISA designed to quantify Human IL-10 with a sensitivity of 5 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 12.5 - 400 pg/mL
- Cited in over 30 publications

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

2 Images

Sandwich ELISA - Human IL-10 ELISA Kit (AB46034)

sELISA

Unknown

Sandwich ELISA - Human IL-10 ELISA Kit (AB46034)

Representative standard curve using ab46034

sELISA

Unknown

Sandwich ELISA - Human IL-10 ELISA Kit (AB46034)

IL-10 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297), with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Results shown after background signal was subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

12.5 - 400 pg/mL

Assay time

2h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-10 ELISA Kit ab46034 is a sandwich ELISA to measure Human IL-10 in serum, plasma, cell culture supernatant with a sensitivity of 5 pg/ml.

How the assay works

A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human IL-10 ELISA Kit ab46034 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution
7. Immediately begin recording the color development

Get results in 90 minutes with our SimpleStep ELISA^® kit

Human IL-10 ELISA Kit (ab185986)

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46034-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46034-CMP04" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46034-CMP13" }, { "size":"4 x 1 Vial", "name":"IL-10 Standard (Lyophilized)", "number":"AB46034-CMP11" }, { "size":"2 x 400 µL", "name":"Biotinylated anti IL-10", "number":"AB46034-CMP02" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46034-CMP01" }, { "size":"4 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46034-CMP09" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46034-CMP06" }, { "size":"2 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB46034-CMP10" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46034-CMP12" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46034-CMP05" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46034-CMP08" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46034-CMP07" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46034-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46034-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46034-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46034-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-10 Standard (Lyophilized)", "number":"AB46034-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB46034-CMP10", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46034-CMP05", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46034-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46034-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46034-CMP06", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46034-CMP09", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti IL-10", "number":"AB46034-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46034-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46034-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46034-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information IL10

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 15: PubMed40428204

2025

Integrating the Interleukins in the Biomarker Panel for the Diagnosis and Prognosis of Patients with Acute Coronary Syndromes: Unraveling a Multifaceted Conundrum.

Applications

Unspecified application

Species

Unspecified reactive species

Amalia-Stefana Timpau,Egidia-Gabriela Miftode,Irina-Iuliana Costache-Enache,Antoniu-Octavian Petris,Ionela-Larisa Miftode,Ivona Mitu,Radu-Stefan Miftode

Scientific reports 15:10804 PubMed40155664

2025

Hepatotoxicity evaluation method through multiple-factor analysis using human pluripotent stem cell derived hepatic organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Dae-Seop Shin,Jung Yoon Yang,Ha Neul Jeong,Seon Ju Mun,Hyunwoo Kim,Myung Jin Son,Myung Ae Bae

International journal of molecular sciences 26: PubMed39795937

2025

Effective Targeting of Glutamine Synthetase with Amino Acid Analogs as a Novel Therapeutic Approach in Breast Cancer.

Applications

Unspecified application

Species

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Shimaa Abdelsattar,Hiba S Al-Amodi,Hala F Kamel,Ahood A Al-Eidan,Marwa M Mahfouz,Kareem El Khashab,Amany M Elshamy,Mohamed S Basiouny,Mohamed A Khalil,Khaled A Elawdan,Shorouk Elsaka,Salwa E Mohamed,Hany Khalil

International journal of molecular sciences 25: PubMed39519098

2024

Pancreatic Adenocarcinoma Up-Regulated Factor (PAUF) Transforms Human Monocytes into Alternative M2 Macrophages with Immunosuppressive Action.

Applications

Unspecified application

Species

Unspecified reactive species

Yeon Jeong Kim,Sitansu Sekhar Nanda,Fen Jiang,Seung Yeon Pyo,Jin-Yeong Han,Sang Seok Koh,Tae Heung Kang

Allergologia et immunopathologia 52:29-35 PubMed39278848

2024

Isoorientin alleviates ovalbumin-stimulated allergic rhinitis in mice by restoring Th1/Th2 balance.

Applications

Unspecified application

Species

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Jianyi Huang,Ran Ji,Xueshen Qian,Yuli Shen

Frontiers in nutrition 10:1164469 PubMed37457975

2023

Eicosapentaenoic acid and branched-chain amino acids fortified complete nutrition drink improved muscle strength in older individuals with inadequate protein intake.

Applications

Unspecified application

Species

Unspecified reactive species

Watcharapol Khoonin,Prapimporn Chattranukulchai Shantavasinkul,Chalat Santivarangkna,Kemika Praengam,Dunyaporn Trachootham

Frontiers in bioengineering and biotechnology 11:1159819 PubMed37346794

2023

Dual CSF1R inhibition and CD40 activation demonstrates anti-tumor activity in a 3D macrophage- HER2 breast cancer spheroid model.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Rodriguez-Perdigon,Laetitia Haeni,Barbara Rothen-Rutishauser,Curzio Rüegg

Molecular biology reports 50:5755-5765 PubMed37219669

2023

The size of human subcutaneous adipocytes, but not adiposity, is associated with inflammation, endoplasmic reticulum stress, and insulin resistance markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Pourdashti,Nassim Faridi,Forouzandeh Monem-Homaie,S Hamid Yaghooti,Ahmadreza Soroush,S Zahra Bathaie

Journal of cellular physiology 239:e31027 PubMed37099691

2023

Sirtuin 6 ameliorates bleomycin-induced pulmonary fibrosis via activation of lipid catabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangping He,Cong Yu,Yunlong Shen,Jiao Huang,Yanzi Zhou,Jianmin Gu,Ying Cao,Quan Zheng

International journal of biological sciences 19:1163-1177 PubMed36923935

2023

Lipocalin-2 promotes acute lung inflammation and oxidative stress by enhancing macrophage iron accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeong Seok An,Jung-Wan Yoo,Jong Hwan Jeong,Manbong Heo,Si Hwan Hwang,Hye Min Jang,Eun Ae Jeong,Jaewoong Lee,Hyun Joo Shin,Kyung Eun Kim,Meong Cheol Shin,Gu Seob Roh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-10 ELISA Kit

Sandwich ELISA - Human IL-10 ELISA Kit (AB46034)

Sandwich ELISA - Human IL-10 ELISA Kit (AB46034)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (35)

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Product promise