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Human IL-10 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IL-10 with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

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Images

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436), expandable thumbnail
  • Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436), expandable thumbnail
  • Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436), expandable thumbnail

Key facts

Detection method
Fluorescent
Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
2.3 - 4800 pg/mL
Assay time
1h 30m
Sensitivity
= 2.1 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

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Target data

Function

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
100X Stoplight Red Substrate
1 x 120 µL
10X Human IL-10 Capture Antibody
1 x 600 µL
10X Human IL-10 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
500X Hydrogen Peroxide (H2O2, 3%)
1 x 50 µL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
Antibody Diluent CPI - HAMA Blocker (ab193969)
1 x 6 mL
Human IL-10 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NBP
1 x 20 mL
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated Black 96-Well Microplate
1 x 1 Unit
Stoplight Red Substrate Buffer
1 x 12 mL

Recommended products

Human IL-10 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IL-10 with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

Key facts

Detection method
Fluorescent
Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
2.3 - 4800 pg/mL
Assay time
1h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 2.1 pg/mL

Precision

Intra assay

Sample
Supernatant
n
5
C.V.
5.2

Inter assay

Sample
Supernatant
n
3
C.V.
3.2

Recovery

Sample specific recovery

Sample type
Cell culture supernatant
Average %
= 108
Range
105 - 111 %
Sample type
Serum
Average %
= 109
Range
108 - 112 %
Sample type
EDTA Plasma
Average %
= 109
Range
103 - 118 %
Sample type
Heparin Plasma
Average %
= 116
Range
113 - 117 %
Sample type
Citrate plasma
Average %
= 111
Range
105 - 119 %
Sample type
Cell culture media
Average %
= 106
Range
102 - 111 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

IL-10 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 protein in human serum, plasma, and cell culture supernatant samples

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Interleukin 10 (IL-10) is a cytokine involved in immunoregulation through the promotion of Th2 response and inhibition of Th1 responses. It is produced by cells of hematopoietic origin, keratinocytes, placental cytotrophoblasts, and hepatic stellate cells.

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3 product images

  • Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (ab229436), expandable thumbnail

    Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (ab229436)

    Human IL-10 Standard Curve Comparison.

    Standard Curve comparison between human IL-10 current (new) SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.

  • Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (ab229436), expandable thumbnail

    Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (ab229436)

    Example of human IL-10 standard curve in Sample Diluent NS + Enhancer.

    The IL-10 standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (ab229436), expandable thumbnail

    Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (ab229436)

    Interpolated concentrations of native IL-10 in human PBMC cell culture supernatant samples.

    The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 462 pg/ml in stimulated PBMC cell culture supernatant. PBMC cell culture supernatant samples were stimulated for 46 hours with 1.5% PHA. Unstimulated PBMC cell culture supernatant samples were also measured; all values were below the detectable range of the assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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