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AB46059

Human IL-10 ELISA Kit High Sensitivity

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(5 Publications)

Human IL-10 ELISA Kit High Sensitivity is a Sandwich (quantitative) ELISA for the measurement of Human IL-10 High Sensitivity in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

1 Images
Sandwich ELISA - Human IL-10 ELISA Kit High Sensitivity (AB46059)
  • sELISA

Unknown

Sandwich ELISA - Human IL-10 ELISA Kit High Sensitivity (AB46059)

Representative Standard Curve using ab46059

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.98 pg/mL

Range

1.56 - 50 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-10 Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in Human sera, plasmas, buffered solutions and cell culture media.

A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-10.

Get results in 90 minutes with Human IL-10 ELISA Kit (ab185986) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 94 %", "average": "= 94" }, { "sample": "Serum", "range": "59 - 93 %", "average": "= 80" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46059-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Amplification Diluent", "number":"AB46059-CMP12", "productcode":"" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46059-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46059-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46059-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10 Microplate (12 x 8 well strips)", "number":"AB46059-CMP10", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti IL-10", "number":"AB46059-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-10 Standard (Lyophilized)", "number":"AB46059-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"Amplifier", "number":"AB46059-CMP11", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46059-CMP08", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46059-CMP01", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46059-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46059-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
See full target information IL10
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 14:89 PubMed35842682

2022

Psychostimulant-induced aberrant DNA methylation in an in vitro model of human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kaili Anier,Kelli Somelar,Külli Jaako,Margret Alttoa,Kerli Sikk,Raul Kokassaar,Kai Kisand,Anti Kalda

Turkish journal of haematology : official journal 34:254-257 PubMed28148470

2017

Diagnostic Accuracy of Interleukin-6, Interleukin-8, and Interleukin-10 for Predicting Bacteremia in Children with Febrile Neutropenia.

Applications

Unspecified application

Species

Unspecified reactive species

Zümrüt Şahbudak Bal,Nihal Karadaş Özdemir,Semra Şen,Deniz Yılmaz Karapınar,Elif Azarsız,Şöhret Aydemir,Fadıl Vardar

PloS one 11:e0164269 PubMed27711256

2016

The Effect of Gender on Mesenchymal Stem Cell (MSC) Efficacy in Neonatal Hyperoxia-Induced Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ibrahim Sammour,Santhosh Somashekar,Jian Huang,Sunil Batlahally,Matthew Breton,Krystalenia Valasaki,Aisha Khan,Shu Wu,Karen C Young

Journal of gastroenterology 52:432-443 PubMed27178566

2016

Efficacy of double-coated probiotics for irritable bowel syndrome: a randomized double-blind controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungsun Han,Jinghwa Wang,Jae-Gu Seo,Hojun Kim

PloS one 8:e77308 PubMed24116222

2013

Chafuroside B, an Oolong tea polyphenol, ameliorates UVB-induced DNA damage and generation of photo-immunosuppression related mediators in human keratinocytes.

Applications

sELISA

Species

Human

Tatsuya Hasegawa,Shoichiro Shimada,Hitoshi Ishida,Masaya Nakashima
View all publications
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websiteProtocolBooklet
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