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Human IL-10 ELISA Kit (Interleukin-10) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-10 (Interleukin-10) in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
9.4 - 3000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human IL-10 ELISA Kit (Interleukin-10) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-10 (Interleukin-10) in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Alternative names=Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
9.4 - 3000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 5 | mean - | SD - | C.V. 5.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 108 | Range 105 - 111 % |
Sample type Serum | Average % = 109 | Range 108 - 112 % |
Sample type EDTA Plasma | Average % = 109 | Range 103 - 118 % |
Sample type Heparin Plasma | Average % = 116 | Range 113 - 117 % |
Sample type Citrate plasma | Average % = 111 | Range 105 - 119 % |
Sample type Cell culture media | Average % = 106 | Range 102 - 111 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human IL-10 ELISA Kit (Interleukin-10) (ab185986) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-10 (Interleukin-10) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-10 (Interleukin-10) with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin 10 (IL-10) is a cytokine involved in immunoregulation through the promotion of Th2 response and inhibition of Th1 responses. It is produced by cells of hematopoietic origin, keratinocytes, placental cytotrophoblasts, and hepatic stellate cells.
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