Human IL-10 ELISA kit (Interleukin-10) is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human IL-10 with a sensitivity of 1.4 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450 nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Broad sample compatibility – works with serum, plasma, and cell culture supernatants
- Cited in over 30 publications
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Publications
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- Scientific reports 13:211802023Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasoumeh Sadat Mousavi Maleki,Ramin Ebrahimi Kiasari,Seyed Javad Seyed Mousavi,Hamid Hashemi-Moghaddam,Ali Akbar Shabani,Hamid Madanchi,Soroush SardariPubMed 38040898
- Nature communications 14:76392023Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun HuangPubMed 37993431
- Frontiers in microbiology 14:12360122023Differential expression analysis of miRNAs in macrophage-derived exosomes in the tuberculosis-infected bone microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhicheng Sun,Xiaoyang Pang,Xiyang Wang,Hao ZengPubMed 37601387
- Nature communications 14:36202023Single-cell transcriptomics and epigenomics unravel the role of monocytes in neuroblastoma bone marrow metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrfete S Fetahu,Wolfgang Esser-Skala,Rohit Dnyansagar,Samuel Sindelar,Fikret Rifatbegovic,Andrea Bileck,Lukas Skos,Eva Bozsaky,Daria Lazic,Lisa Shaw,Marcus Tötzl,Dora Tarlungeanu,Marie Bernkopf,Magdalena Rados,Wolfgang Weninger,Eleni M Tomazou,Christoph Bock,Christopher Gerner,Ruth Ladenstein,Matthias Farlik,Nikolaus Fortelny,Sabine Taschner-MandlPubMed 37365178
- Experimental and therapeutic medicine 26:3592023CX3CL1/CX3CR1 axis alleviates inflammation and apoptosis in human nucleus pulpous cells via M2 macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Wen Gao,Han-Lin Hu,Ming-Hua Xie,Cai-Xia Tang,Jun Ou,Zheng-Hao LuPubMed 37324510
- Cell death discovery 9:922023Midkine inhibition enhances anti-PD-1 immunotherapy in sorafenib-treated hepatocellular carcinoma via preventing immunosuppressive MDSCs infiltration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijuan Ding,Nanya Wang,Qiang Wang,Xia Fan,Yuning Xin,Shudong WangPubMed 36906597
- Nutrients 15:2023The Effect of Weekly 50,000 IU Vitamin D Supplements on the Serum Levels of Selected Cytokines Involved in Cytokine Storm: A Randomized Clinical Trial in Adults with Vitamin D Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDana A Bader,Anas Abed,Beisan A Mohammad,Ahmad Aljaberi,Ahmad Sundookah,Maha Habash,Ahmad R Alsayed,Mohammad Abusamak,Sami Al-Shakhshir,Mahmoud Abu-SamakPubMed 36904187
- Medicine 102:e323842023Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGui Yang,Youjiang QiuPubMed 36820569
- International journal of molecular sciences 24:2022Bacterial DNAemia in Alzheimer's Disease and Mild Cognitive Impairment: Association with Cognitive Decline, Plasma BDNF Levels, and Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobertina Giacconi,Patrizia D'Aquila,Marta Balietti,Cinzia Giuli,Marco Malavolta,Francesco Piacenza,Laura Costarelli,Demetrio Postacchini,Giuseppe Passarino,Dina Bellizzi,Mauro ProvincialiPubMed 36613538
- Frontiers in endocrinology 13:6578022022Downregulationof circ_0001578 promotes gestational diabetes mellitus by inducing placental inflammation the NF-κB and JNKs pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhang,Xudong Zhao,Ling LiPubMed 36263320
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 9.4 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
Alternative names
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10
What's included?
Recommended products
Human IL-10 ELISA kit (Interleukin-10) is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human IL-10 with a sensitivity of 1.4 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450 nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Broad sample compatibility – works with serum, plasma, and cell culture supernatants
- Cited in over 30 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 9.4 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.4 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 5 | C.V. 5.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 3 | C.V. 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 108 | Range 105 - 111 % |
Sample type Serum | Average % = 109 | Range 108 - 112 % |
Sample type EDTA Plasma | Average % = 109 | Range 103 - 118 % |
Sample type Heparin Plasma | Average % = 116 | Range 113 - 117 % |
Sample type Citrate plasma | Average % = 111 | Range 105 - 119 % |
Sample type Cell culture media | Average % = 106 | Range 102 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-10 ELISA Kit (Interleukin-10) ab185986 is a rapid single-wash 90-min ELISA kit to measure Human IL-10 in cell culture supernatant, serum, plasma. This SimpleStep ELISA® sensitivity is 1.4 pg/mL.
How the assay works
Human IL-10 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human IL-10. The standard protein in this kit is mature full length human IL-10. Native signal was detected in cell culture supernatant. Spike protein experiments were used to validate serum and plasma samples. Saliva, milk, CSF, cell extract and tissue extract samples have not bee tested with this kit. 50% pooled serum and plasma samples from healthy donors were measured in duplicate. All values were below the detectable range of this assay. 50% serum from 10 healthy human male donors was measured in duplicate. All values were below the detectable range of this assay.
Human IL-10 ELISA Kit (Interleukin-10) ab185986 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IL-10 ELISA Kit ab185986
Human IL-10 ELISA Kit ab185986 has been used to assess inflammation in different physio-pathological contexts such as celiac disease(1), tuberculosis(2), and atherosclerosis(3).
References:
(1)Soroush Sardari et al. 2023, PMID: 38040898,
(2)Hao Zeng et al. 2023, PMID: 37601387,
(3)Youjiang Qiu et al. 2023, PMID: 36820569.
Save your precious samples
Our 384-well Human IL-10 ELISA Kit (Interleukin-10) only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human IL-10 Antibody Pair - BSA and Azide free (Interleukin-10) Human IL-10 Antibody Pair - BSA and Azide free (Interleukin-10) ab243976
- Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture) Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture) ab244835
- Anti-IL-10 antibody [EPR20433-290] - BSA and Azide free (Detector) Anti-IL-10 antibody [EPR20433-290] - BSA and Azide free (Detector) ab244889
Related and recommended products
Human IL-10 ELISA Kit ab185986 is commonly used to measure IL-10 as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit Human TNF alpha ELISA Kit ab181421
- Human IL-8 ELISA kit Human IL-8 ELISA Kit ab214030
- Human IL-6 ELISA kit Human IL-6 ELISA Kit ab178013
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
KO data.
Human IL-10 concentration was interpolated from the IL-10 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-10 ELISA Kit (Interleukin-10) (ab185986). Wild-type and IL-10 knockout THP-1 (ab273763) vehicle control and PMA (100 ng/mL, 56 h) + LPS (1 μg/mL, 24 h) treated cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation."
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Human IL-10 Standard Curve Comparison.
Standard Curve comparison between human IL-10 current (new) SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Example of human IL-10 standard curve in Sample Diluent 50BP + Enhancer.
The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Example of human IL-10 standard curve in Sample Diluent NS + Enhancer.
The IL-10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Interpolated concentrations of native IL-10 in human PBMC cell culture supernatant samples.
The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 462 pg/ml in stimulated PBMC cell culture supernatant. PBMC cell culture supernatant samples were stimulated for 46 hours with 1.5% PHA. Unstimulated PBMC cell culture supernatant samples were also measured; all values were below the detectable range of the assay.
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Interpolated concentration of native IL-10 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% stimulated PBMC supernatant (1.5% PHA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Example of human IL-10 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-10 ELISA Kit (Interleukin-10) (ab185986)
Example of human IL-10 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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