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Human IL-10 RA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-10 RA in Human in Serum, Plasma, Cell culture supernatant samples.

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Images

Sandwich ELISA - Human IL-10 RA ELISA Kit (AB314734), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
2.5 - 500 ng/mL
Sensitivity
= 2.5 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683).

Alternative names

CD210, IL10R, IL10RA, Interleukin-10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
500X HRP-Streptavidin
1 x 200 µL
5X Assay Diluent B (Item E)
1 x 15 mL
Assay Diluent C (Item L)
1 x 30 mL
Biotinylated Human IL-10 RA Detection Antibody
2 x 1 Vial
Human IL-10 RA Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human IL-10 RA Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human IL-10 RA ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-10 RA in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
2.5 - 500 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 2.5 ng/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 89.97
Range
83 - 96 %
Sample type
Plasma
Average %
= 89.96
Range
80 - 95 %
Sample type
Cell culture media
Average %
= 99.67
Range
90 - 108 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human IL-10 RA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-10 RA in serum, plasma (human IL-10 RA concentration is low in normal serum/plasma and may not be detectable in this assay), and cell culture supernatants. This assay employs an antibody specific for human IL-10 RA coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human IL-10 RA ELISA Kit (ab314734), expandable thumbnail

    Sandwich ELISA - Human IL-10 RA ELISA Kit (ab314734)

    Example of standard curve using ab314734. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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