JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314734

Human IL-10 RA ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human IL-10 RA ELISA Kit is a Sandwich ELISA for the measurement of Human IL-10 RA in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD210, IL10R, IL10RA, Interleukin-10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1

1 Images
Sandwich ELISA - Human IL-10 RA ELISA Kit (AB314734)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-10 RA ELISA Kit (AB314734)

Example of standard curve using ab314734. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.5 ng/mL

Range

2.5 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB197666

Anti-IL-10RA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10RA antibody (AB197666)

  • 0
  • 0
Proteins & Peptides

AB214604

Recombinant human IL-10RA protein (Fc Chimera)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human IL-10 RA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-10 RA in serum, plasma (human IL-10 RA concentration is low in normal serum/plasma and may not be detectable in this assay), and cell culture supernatants. This assay employs an antibody specific for human IL-10 RA coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL5, IL-6, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF,SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

style
left-column

Recovery

[ { "sample": "Serum", "range": "83 - 96 %", "average": "= 89.97" }, { "sample": "Plasma", "range": "80 - 95 %", "average": "= 89.96" }, { "sample": "Cell culture media", "range": "90 - 108 %", "average": "= 99.67" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314734-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human IL-10 RA Detection Antibody", "number":"AB314734-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB314734-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314734-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human IL-10 RA Protein Standard", "number":"AB314734-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314734-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB314734-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314734-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314734-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human IL-10 RA Antibody-coated ELISA Plate", "number":"AB314734-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed : 16982608). In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed : 12133952). These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed : 26962683).
See full target information IL10RA
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com