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AB100550

Human IL-10RB ELISA Kit

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Human IL-10RB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-10RB in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CDw210b, CRFB4, D21S58, D21S66, IL10RB, Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, CRF2-4, IL-10R subunit 2, IL-10R2

2 Images
Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)
  • sELISA

Unknown

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)

Representative standard curve using ab100550

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)
  • sELISA

Unknown

Sandwich ELISA - Human IL-10RB ELISA Kit (AB100550)

Representative standard curve using ab100550

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

4.12 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-10RB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10RB in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human IL-10RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "120 - 140 %", "average": "= 129.1" }, { "sample": "Serum", "range": "110 - 129 %", "average": "= 121.9" }, { "sample": "Plasma", "range": "92 - 112 %", "average": "= 104.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100550-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100550-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-10RB", "number":"AB100550-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100550-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100550-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100550-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100550-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100550-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-10RB Standard (lyophilized)", "number":"AB100550-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-10RB Microplate (12 x 8 wells)", "number":"AB100550-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Shared cell surface receptor required for the activation of five class 2 cytokines : IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
See full target information IL10RB
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websiteProtocolBooklet
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Product promise

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