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AB243672

Human IL-10RB ELISA Kit

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Human IL-10RB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-10RB with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CDw210b, CRFB4, D21S58, D21S66, IL10RB, Interleukin-10 receptor subunit beta, IL-10 receptor subunit beta, IL-10R subunit beta, IL-10RB, Cytokine receptor class-II member 4, Cytokine receptor family 2 member 4, Interleukin-10 receptor subunit 2, CRF2-4, IL-10R subunit 2, IL-10R2

2 Images
Sandwich ELISA - Human IL-10RB ELISA Kit (AB243672)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-10RB ELISA Kit (AB243672)

Interpolated concentrations of native IL-10RB in human cell extract based on a 162.5 μg/mL extract load.

The concentrations of IL-10RB were measured in duplicate and interpolated from the IL-10RB standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10RB concentration was determined to be 360.1 pg/mL.

Sandwich ELISA - Human IL-10RB ELISA Kit (AB243672)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-10RB ELISA Kit (AB243672)

Example of human IL-10RB standard curve in 1X Cell Extraction Buffer PTR.

The IL-10RB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-10RB ELISA Kit (ab243672) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-10RB protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-10RB with 1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "87 - 139 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243672-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243672-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB243672-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243672-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243672-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-10RB Capture Antibody", "number":"AB243672-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-10RB Lyophilized Recombinant Protein", "number":"AB243672-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243672-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB243672-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-10RB Detector Antibody", "number":"AB243672-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243672-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243672-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Shared cell surface receptor required for the activation of five class 2 cytokines : IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
See full target information IL10RB
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websiteProtocolBooklet
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