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AB189569

Human IL-11 ELISA Kit

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(6 Publications)

Human IL-11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-11 with a sensitivity of 83.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations

View Alternative Names

Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11

5 Images
ELISA - Human IL-11 ELISA Kit (AB189569)
  • ELISA

Supplier Data

ELISA - Human IL-11 ELISA Kit (AB189569)
Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)

Example of IL-11 standard curve for cell culture supernatant samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)

Titration of PMA + IL-1 alpha - treated MRC5 supernatant within the working range of the assay.

MRC5 cells were grown in 10F HGDMEM media in the presence of 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 ng/mL IL-1 alpha (ab9616) for 48 hours. IL-11 concentrations were measured in serial dilutions of cell culture supernatants using this kit. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)

Comparison of IL-11 concentrations in untreated and PMA + IL-1 alpha - treated MRC5 supernatants. MRC5 cells were grown in 10F HGDMEM media in the absence or presence of 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 ng/mL IL-1 alpha (ab9616) for 48 hours. IL-11 concentrations were measured in 8 X diluted cell culture supernatants using this kit. Interpolated data values adjusted for sample dilution are graphed (mean +/- SD, n=3) in ng of IL-11 per mL of cell culture supernatant.

Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB189569)

Example of IL-11 standard curve for serum and plasma (EDTA) samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 83.3 pg/mL

Range

375 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-11 ELISA Kit ab189569 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-11 in cell culture supernatant, EDTA plasma, serum. This SimpleStep sensitivity is 83.3 pg/mL.

How the assay works

Human IL-11 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-11 ELISA Kit ab189569 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

IL-11 is multifunctional cytokine of IL-6 superfamily. It is secreted by many cell types. IL-11 is best known for its hematopoietic activity. IL-11directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. During embryonic development L-11 signaling is required for normal development of placentation and survival to birth.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" } ]
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Recovery

[ { "sample": "Serum", "range": "94.9 - 104.4 %", "average": "= 99.6" }, { "sample": "EDTA Plasma", "range": "108.3 - 125.3 %", "average": "= 116.9" }, { "sample": "Cell culture media", "range": "93.5 - 104.3 %", "average": "= 98.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab189569-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB189569-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB189569-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB189569-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB189569-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB189569-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB189569-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB189569-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-11 Capture Antibody", "number":"AB189569-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB189569-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-11 Lyophilized Recombinant Protein", "number":"AB189569-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-11 Detector Antibody", "number":"AB189569-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed : 12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed : 30279168).
See full target information IL11
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39408993

2024

Radiofrequency Currents Modulate Inflammatory Processes in Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Toledano-Macías,María Antonia Martínez-Pascual,Almudena Cecilia-Matilla,Mariano Bermejo-Martínez,Alfonso Pérez-González,Rosa Cristina Jara,Silvia Sacristán,María Luisa Hernández-Bule

Frontiers in oncology 10:305 PubMed32269963

2020

CaSR Induces Osteoclast Differentiation and Promotes Bone Metastasis in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lian Liu,Yichang Fan,Zhaoxin Chen,Yujian Zhang,Jing Yu

Frontiers in immunology 10:2312 PubMed31636637

2019

Setmelanotide, a Novel, Selective Melanocortin Receptor-4 Agonist Exerts Anti-inflammatory Actions in Astrocytes and Promotes an Anti-inflammatory Macrophage Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Alwin Kamermans,Tom Verhoeven,Bert van Het Hof,Jasper J Koning,Lauri Borghuis,Maarten Witte,Jack van Horssen,Helga E de Vries,Merel Rijnsburger

International journal of clinical and experimental 12:2595-2603 PubMed31934087

2019

Long non-coding RNA NKILA inhibits proliferation and migration of lung cancer via IL-11/STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Liu,Xiuyan Shi

Oncotarget 8:76189-76203 PubMed29100303

2017

KRT8 upregulation promotes tumor metastasis and is predictive of a poor prognosis in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Song Tan,Wei-Hua Jiang,Yi He,De-Sheng Wang,Zhen-Jie Wu,Deng-Shuang Wu,Li Gao,Yi Bao,Jia-Zi Shi,Bing Liu,Li-Jun Ma,Lin-Hui Wang

Scientific reports 6:37070 PubMed27901021

2016

TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling.

Applications

WB

Species

Human

Hao Zheng,Yuan Yang,Jun Han,Wei-Hua Jiang,Cheng Chen,Meng-Chao Wang,Rong Gao,Shuai Li,Tao Tian,Jian Wang,Li-Jun Ma,Hao Ren,Wei-Ping Zhou
View all publications
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websiteProtocolBooklet
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Product promise

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