AB324512

Human IL-11 ELISA Kit

SimpleStep
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Human IL-11 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-11 in Human in Biofluids, Other samples samples.

View Alternative Names

Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11

7 Images

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 15% treated MCR-5 cell culture supernatant (treated with 10 ng/mL PMA + 1 ng/mL IL-1 alpha for 48 hrs) and 60% untreated MCR-5 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No signal was observed in neat cell culture media (DMEM containing 10% fetal bovine serum).

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A549 cell culture supernatant 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Example of human IL-11 standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Example of human IL-11 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Standard curve comparison between Human IL-11 SimpleStep ELISA kit (ab324512) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Standard curve comparison between the original Human IL-11 SimpleStep ELISA (ab189569) and current Human IL-11 SimpleStep ELISA (ab324512).

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 15% treated MCR-5 cell culture supernatant (treated with 10 ng/mL PMA + 1 ng/mL IL-1 alpha for 48 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 17.38 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-11 with a sensitivity of 17.38pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" } ]

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Recovery

[ { "sample": "Serum", "range": "93 - 100 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "88 - 110 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "83 - 85 %", "average": "= 84" }, { "sample": "Cell culture supernatant", "range": "93 - 99 %", "average": "= 96" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed : 12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed : 30279168).

See full target information IL11

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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