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AB326005

Human IL-11 ELISA Kit, Chemiluminescent

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Human IL-11 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IL-11 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 6.86 - 15000 pg/ml with a sensitivity of 4.5 pg/mL.
- Patent pending

View Alternative Names

Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11

5 Images
Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)

Standard curve comparison between the Chemiluminescent Human IL-11 SimpleStep ELISA and colorimetric Human IL-11 SimpleStep ELISA (ab324512) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)

Dynamic range comparison between the Chemiluminescent Human IL-11 SimpleStep ELISA, colorimetric Human IL-11 SimpleStep ELISA (ab324512), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 15% treated MCR-5 cell culture supernatant (treated with 10 ng/mL PMA + 1 ng/mL IL-1 alpha for 48 hrs) and 60% untreated MCR-5 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No signal was observed in neat cell culture media (DMEM containing 10% fetal bovine serum).

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)
  • sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit, Chemiluminescent (AB326005)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A549 cell culture supernatant 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4.5 pg/mL

Range

6.86 - 15000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human IL-11 ELISA Kit, Chemiluminescent (ab326005) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-11 in Serum, Citrate Plasma, EDTA Plasma, Cell Culture Supernatant with a sensitivity of 4.5 pg/mL.

How the assay works

Human IL-11 ELISA Kit, Chemiluminescent (ab326005) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-11 (ab326005) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 100 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "88 - 110 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "83 - 85 %", "average": "= 84" }, { "sample": "Cell culture supernatant", "range": "93 - 99 %", "average": "= 96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326005-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326005-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB326005-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326005-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-11 Lyophilized Recombinant Protein", "number":"AB326005-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326005-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-11 Detector Antibody", "number":"AB326005-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-11 Capture Antibody", "number":"AB326005-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326005-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326005-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326005-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed : 12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed : 30279168).
See full target information IL11
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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