Human IL-12 ELISA Kit High Sensitivity

Product details

Human IL-12 ELISA Kit High Sensitivity ab46035 is a sandwich ELISA to measure Human IL-12 High Sensitivity in serum, plasma, cell culture supernatant with a sensitivity of 0.75 pg/ml.

How the assay works

Human IL-12 ELISA kit High Sensitivity is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-12.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human IL-12 ELISA Kit High Sensitivity ab46035 protocol summary

1. Add standard or sample to each well used.
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature.
3.Aspirate and wash each well.
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature.
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution.
7. Immediately begin recording the color development