Human IL-12/IL-23 p40 ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Human IL-12/IL-23 p40 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-12/IL-23 p40 with a sensitivity of 5.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40
- sELISA
Supplier Data
Sandwich ELISA - Human IL-12/IL-23 p40 ELISA Kit (AB220656)
Interpolated concentrations of native IL-12/IL-23P40 in human serum samples.
Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-12/IL-23P40 concentration was determined to be 92 pg/mL with a range of 17 – 100 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human IL-12/IL-23 p40 ELISA Kit (AB220656)
Interpolated concentrations of native IL-12/IL-23P40 in human PMBC and THP1 stimulated cell culture supernatant samples.
The concentrations of IL-12/IL-23P40 was measured in duplicate, interpolated from the IL-12/IL-23P40 standard curve and corrected for sample dilution. Undiluted samples are as follows : Stimulated PBMC cell culture supernatant (50%) and Stimulated THP1 cell culture supernatant (12.5%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-12/IL-23P40 concentration was determined to be 369 pg/mL in Stimulated PBMC Cell culture supernatant and 10,253 pg/mL in THP1 cell culture supernatant. IL-12/IL-23P40 was undetectable in media or unstimulated supernatant samples.
- sELISA
Supplier Data
Sandwich ELISA - Human IL-12/IL-23 p40 ELISA Kit (AB220656)
Interpolated concentrations of spiked IL-12/IL-23P40 in human serum and plasma samples.
The concentration of IL-12/IL-23P40 was measured in duplicate, interpolated from the IL-12/IL-23P40 standard curve and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human IL-12/IL-23 p40 ELISA Kit (AB220656)
Interpolated concentrations of native IL-12/IL-23P40 in human serum and plasma samples.
The concentrations of IL-12/IL-23P40 were measured in duplicate, interpolated from the IL-12/IL-23P40 standard curve and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-12/IL-23P40 concentration was determined to be 45 pg/mL in serum, 41 pg/mL in plasma (EDTA), 42 pg/mL in plasma (heparin) and 42 pg/mL in plasma (citrate).
- sELISA
Supplier Data
Sandwich ELISA - Human IL-12/IL-23 p40 ELISA Kit (AB220656)
Example of human IL-12/IL-23P40 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human IL-12/IL-23 p40 ELISA Kit (ab220656) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-12/IL-23 p40 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-12/IL-23 p40 with 5.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-12/IL-23P40 is a 40kDa cytokine that exists in its monomeric form as well as heterodimerizes with a 35kDa subunit to form IL-12/IL-12p70 and heterodimerizes with a 19kDa subunit to form IL-23. IL‑12/IL‑23P40 can act as a growth factor for activated T and natural killer cells as well as stimulate the production of Interferon gamma. IL-12/IL-23P40 is expressed by activated macrophages that induces Th1 cell development. This assay recognizes IL-12/IL-23P40 monomer, as well as the heterodimerized forms.
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Properties and storage information
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Product protocols
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Publications (1)
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Journal of clinical biochemistry and nutrition 72:215-224 PubMed37251958
2023
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