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AB46037

Human IL-12 p40 + IL-12 p70 ELISA Kit

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(1 Publication)

Human IL-12 p40 + IL-12 p70 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-12 p40 + IL-12 p70 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40, NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

1 Images
Sandwich ELISA - Human IL-12 p40 + IL-12 p70 ELISA Kit (AB46037)
  • sELISA

Unknown

Sandwich ELISA - Human IL-12 p40 + IL-12 p70 ELISA Kit (AB46037)

Representative Standard Curve using ab46037

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 20 pg/mL

Range

62.5 - 2000 pg/mL

Assay time

1h 45m

Assay Platform

Microplate

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AB215089

Human IL-4 ELISA Kit

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-12 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and supernatants.

A monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-12, and the Human Il-12 p40 monomer, dimer or heterodimer p70 (p40 + p35) forms of the molecule.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 18, "mean": "2277 pg/mL", "standardDeviation": "160", "coefficientOfVariability": "7" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 18, "mean": "1199 pg/mL", "standardDeviation": "108", "coefficientOfVariability": "9" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 18, "mean": "479 pg/mL", "standardDeviation": "49", "coefficientOfVariability": "10.3" }, { "reproducibilityType": "Inter", "sample": "Sample 4", "replicates": 18, "mean": "1488 pg/mL", "standardDeviation": "123", "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Inter", "sample": "Sample 5", "replicates": 18, "mean": "826 pg/mL", "standardDeviation": "106", "coefficientOfVariability": "12.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
See full target information IL12B

Additional targets

IL12A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:665869 PubMed33937269

2021

Enhancing Fatty Acid Catabolism of Macrophages Within Aberrant Breast Cancer Tumor Microenvironment Can Re-establish Antitumor Function.

Applications

Unspecified application

Species

Unspecified reactive species

Yucui Gu,Xingjian Niu,Lei Yin,Yiran Wang,Yue Yang,Xudong Yang,Qingyuan Zhang,Hongfei Ji
View all publications
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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