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AB46143

Human IL-12 p70 ELISA Kit

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Human IL-12 p70 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-12 p70 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

1 Images
Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB46143)
  • sELISA

Unknown

Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB46143)

Representative Standard Curve using ab46143

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.2 pg/mL

Range

6.25 - 200 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-12 p70 (Interleukin- 12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 in supernatants, buffered solutions or serum and plasma samples.

A monoclonal antibody specific IL-12 p70 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 p70 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 p70 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 p70 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-12 p70.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 94 %", "average": "= 91" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46143-2x96Tests", "assetComponentDetails": [ { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46143-CMP07" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-12 p70", "number":"AB46143-CMP12" }, { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46143-CMP03" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46143-CMP11" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46143-CMP02" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46143-CMP05" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46143-CMP04" }, { "size":"4 x 1 Vial", "name":"IL-12p70 Standard (Lyophilized)", "number":"AB46143-CMP10" }, { "size":"2 x 1 Unit", "name":"IL-12 p70 Microplate (12 x 8 well strips)", "number":"AB46143-CMP09" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46143-CMP08" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46143-CMP06" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46143-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46143-1x96Tests", "assetComponentDetails": [ { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46143-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46143-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46143-CMP11", "productcode":"" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46143-CMP07", "productcode":"" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46143-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46143-CMP05", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-12 p70", "number":"AB46143-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-12 p70 Microplate (12 x 8 well strips)", "number":"AB46143-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-12p70 Standard (Lyophilized)", "number":"AB46143-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46143-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46143-CMP06", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46143-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed : 8605935, PubMed : 8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 1673147, PubMed : 1674604, PubMed : 8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed : 8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed : 7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed : 7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed : 22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed : 22306691).
See full target information IL12A
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websiteProtocolBooklet
en
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Product promise

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