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AB326382

Human IL-12 p70 ELISA Kit, Chemiluminescent

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Human IL-12 p70 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IL-12 p70 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 6.39 - 4660 pg/ml with a sensitivity of 1.83pg/mL.

-Patent pending

View Alternative Names

NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

4 Images
Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)
  • sELISA

Lab

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)
  • sELISA

Lab

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)

Interpolated concentrations of spiked recombinant IL-12 p70 in human serum, plasma and cell culture supernatant samples. The concentrations of IL-12 p70 were measured in duplicate, interpolated from the IL-12 p70 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, plasma (citrate) 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)
  • sELISA

Lab

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)

Dynamic range comparison between the Chemiluminescent Human IL-12 p70 SimpleStep ELISA, colorimetric Human IL-12 p70 SimpleStep ELISA (ab223592), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)
  • sELISA

Lab

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Chemiluminescent (AB326382)

Standard curve comparison between the Chemiluminescent Human IL-12 p70 SimpleStep ELISA and colorimetric Human IL-12 p70 SimpleStep ELISA (ab223592) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.83 pg/mL

Range

6.39 - 4660 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-12 p70 ELISA Kit, Chemiluminescent (ab326382) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-12 p70 in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 1.83 pg/mL.

How the assay works

Human IL-12 p70 ELISA Kit, Chemiluminescent (ab326382) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-12 p70 (ab326382) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.2" } ]
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Recovery

[ { "sample": "Heparin Plasma", "range": "93 - 101 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Serum", "range": "80 - 89 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "92 - 94 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326382-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326382-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326382-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-12 p70 Lyophilized Recombinant Protein", "number":"AB326382-CMP03", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326382-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB326382-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-12 p70 Capture Antibody", "number":"AB326382-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326382-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326382-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326382-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-12 p70 Detector Antibody", "number":"AB326382-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed : 8605935, PubMed : 8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 1673147, PubMed : 1674604, PubMed : 8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed : 8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed : 7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed : 7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed : 22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed : 22306691).
See full target information IL12A
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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