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AB229515

Human IL-12 p70 ELISA Kit, Fluorescent

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Human IL-12 p70 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IL-12 p70 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

NKSF2, IL12B, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, NK cell stimulatory factor chain 2, CLMF p40

2 Images
Sandwich ELISA - Human IL-12 p70 ELISA Kit, Fluorescent (AB229515)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Fluorescent (AB229515)

Interpolated concentrations of spike IL-12 p70 in human serum, plasma and cell culture supernatant samples.

The concentrations of IL-12 p70 were measured in duplicates, interpolated from the IL-12 p70 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, plasma (citrate) 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of IL-12 p70 was measured.

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Fluorescent (AB229515)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-12 p70 ELISA Kit, Fluorescent (AB229515)

Example of human IL-12 p70 standard curve in Sample Diluent NS.

The IL-12 p70 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Fluorescent

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 pg/mL

Range

3.9 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

IL-12 p70 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 protein in human serum, plasma and cell culture supernatant.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

IL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits IL-12A (p35) and IL-12B (p40). This kit recognizes specifically the IL-12 total heterodimer protein. IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFNγ.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 101 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "80 - 89 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "92 - 94 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "94 - 110 %", "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab229515-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB229515-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB229515-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB229515-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-12 p70 Capture Antibody", "number":"AB229515-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI - HAMA Blocker (ab193969)", "number":"AB229515-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-12 p70 Lyophilized Recombinant Protein", "number":"AB229515-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB229515-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-12 p70 Detector Antibody", "number":"AB229515-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB229515-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB229515-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB229515-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
See full target information IL12B
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websiteProtocolBooklet
en
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Product promise

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