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AB303737

Human IL-12RB1 ELISA Kit

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Human IL-12RB1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-12RB1 with a sensitivity of 38.236 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

CD212, IL12R, IL12RB, IL12RB1, Interleukin-12 receptor subunit beta-1, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, IL-12R-beta-1, IL-12RB1, IL-12 receptor beta component

2 Images
Sandwich ELISA - Human IL-12RB1 ELISA Kit (AB303737)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-12RB1 ELISA Kit (AB303737)

Interpolated concentrations of human IL-12RB1 in 293T Cell Extract and IL-12RB1 overexpression 293T extract.

Interpolated concentration of native IL-12RB1 was measured in duplicate at different sample concentrations. Undiluted samples are 250 ng/mL 293T Cell Extract and IL-12RB1 overexpression 293T extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human IL-12RB1 ELISA Kit (AB303737)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-12RB1 ELISA Kit (AB303737)

Example of human IL-12RB1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 38.236 pg/mL

Range

101.563 - 6500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-12RB1 SimpleStep ELISA® kit (ab303737) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-12RB1 protein in cell lysates. Quantitate Human IL-12RB1 with 38.236 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.53" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.26" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "98 - 103 %", "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303737-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303737-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303737-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS", "number":"AB303737-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB303737-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB303737-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-12RB1 Capture Antibody", "number":"AB303737-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-12RB1 Lyophilized Recombinant Protein", "number":"AB303737-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB303737-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-12RB1 Detector Antibody", "number":"AB303737-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-Coated 96-Well Microplate", "number":"AB303737-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB303737-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
See full target information IL12RB1
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websiteProtocolBooklet
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Product promise

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