Human IL-12RB1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-12RB1 with a sensitivity of 38.236 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 101.563 - 6500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 38.236 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.
Alternative names
CD212, IL12R, IL12RB, IL12RB1, Interleukin-12 receptor subunit beta-1, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, IL-12R-beta-1, IL-12RB1, IL-12 receptor beta component
What's included?
Recommended products
Human IL-12RB1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-12RB1 with a sensitivity of 38.236 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 101.563 - 6500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 38.236 pg/mL
- General recovery
- 101%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 5.26 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 8.53 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 101 | Range 98 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-12RB1 SimpleStep ELISA® kit (ab303737) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-12RB1 protein in cell lysates. Quantitate Human IL-12RB1 with 38.236 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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2 product images
Sandwich ELISA - Human IL-12RB1 ELISA Kit (ab303737)
Example of human IL-12RB1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-12RB1 ELISA Kit (ab303737)
Interpolated concentration of native IL-12RB1 was measured in duplicate at different sample concentrations. Undiluted samples are 250 ng/mL 293T Cell Extract and IL-12RB1 overexpression 293T extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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