AB288591

Human IL-13 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human IL-13 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-13 with a sensitivity of 1.662 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NC30, IL13, Interleukin-13, IL-13

5 Images

ELISA - Human IL-13 ELISA Kit (AB288591)

ELISA

Supplier Data

ELISA - Human IL-13 ELISA Kit (AB288591)

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Standard curve comparison between the original Human IL-13 SimpleStep ELISA (ab178014) and current Human IL-13 SimpleStep ELISA (ab288591).

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Interpolated concentration of native IL-13 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Standard curve comparison between Human IL-13 SimpleStep ELISA kit (ab288591) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Example of human IL-13 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.662 pg/mL

Range

4.688 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-13 ELISA Kit ab288591 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-13 in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.662 pg/mL.

How the assay works

Human IL-13 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-13 ELISA Kit ab288591 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-13 antibody [EPR20465-23] - BSA and Azide free (Capture) ab243007
- Anti-IL-13 antibody [EPR20465-21] - BSA and Azide free (Detector) ab243007

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.91" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 104 %", "average": "= 94" }, { "sample": "Serum", "range": "88 - 90 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "91 - 101 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "91 - 97 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "82 - 91 %", "average": "= 86" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288591-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288591-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288591-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288591-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288591-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288591-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB288591-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-13 Lyophilized Recombinant Protein", "number":"AB288591-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-13 Capture Antibody", "number":"AB288591-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-13 Detector Antibody", "number":"AB288591-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288591-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 8096327, PubMed : 8097324). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed : 8096327). Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (PubMed : 7903680, PubMed : 8759755). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (By similarity). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils (PubMed : 8639787). Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 9013879). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 21622864).

See full target information IL13

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:2670-2682 PubMed39006280

2024

Screening and analysis for potential clinical diagnostic and prognostic markers in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yejun Liu,Yonggang Kong,Xuhong Zhou

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-13 ELISA Kit

ELISA - Human IL-13 ELISA Kit (AB288591)

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Sandwich ELISA - Human IL-13 ELISA Kit (AB288591)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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