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AB47353

Human IL-13 ELISA Set (without plates)

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(1 Publication)

Human IL-13 ELISA Set (without plates) is a Sandwich (quantitative) ELISA for the measurement of Human IL-13 ELISA Set (without plates) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NC30, IL13, Interleukin-13, IL-13

1 Images
Sandwich ELISA - Human IL-13 ELISA Set (without plates) (AB47353)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-13 ELISA Set (without plates) (AB47353)

IL-13 Standard curve ranging from 3.12 to 100 pg/mL

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 1.5 pg/mL

Range

3.125 - 100 pg/mL

Assay time

2h 15m

Assay Platform

Reagents

Reactivity data

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Product details

Abcam's IL-13 Human in vitro ELISA Set is designed for the quantitative measurement of IL-13 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

This ELISA Set is intended to measure human IL-13 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

The IL-13 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

Recognizes both natural and recombinant human IL-13.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 8096327, PubMed : 8097324). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed : 8096327). Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (PubMed : 7903680, PubMed : 8759755). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (By similarity). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils (PubMed : 8639787). Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 9013879). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 21622864).
See full target information IL13

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2022:3066456 PubMed35915851

2022

XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Qu,Hongyan Li,Lingzhao Meng
View all publications
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