Human IL-13 RA1 ELISA Kit

Product details

Human IL-13 RA1 ELISA Kit is designed for the quantitative determination of IL-13 RA1 in cell culture supernatants, plasma and serum samples.

Δ Note: Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended as anticoagulants for use in this assay.

This assay employs an antibody specific for human IL-13 RA1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 RA1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-13 RA1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 RA1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human Angiogenin, BDNF Activin A, AgRP, Angiogenin, Angiopoietin-1, Angiostatin, Cathepsin S, CD40 (TNFRSF5), Cripto-1, DAN, DKK-1, ECadherin, Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), Follistatin, Galectin-7, gp130, ICAM-2 (CD102), IL-1 R4 (ST2), IL-13 R alpha 2, IL-17B, IL-2 R alpha, IL-2 R beta (CD122), IL-23, LAP/TGF beta 1, NrCAM, PAI 1, PDGF-AB, Resistin, SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), Sonic Hedgehog NTerminal (Shh-N), TGF beta 2, Thrombopoietin (TPO), Tie-2, TRAIL R4 (TNFRSF10D), TREM-1, TROP1 (EpCAM), VEGF-C, VEGFR1.