AB303763

Human IL-13 receptor alpha 1 ELISA Kit

Recombinant
SimpleStep
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Human IL-13 receptor alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-13 receptor alpha 1 with a sensitivity of 5.939 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD213a1, IL13R, IL13RA, IL13RA1, Interleukin-13 receptor subunit alpha-1, IL-13 receptor subunit alpha-1, IL-13R subunit alpha-1, IL-13R-alpha-1, IL-13RA1, Cancer/testis antigen 19, CT19

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Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of human IL-13 receptor alpha 1 in serum, plasma – citrate, plasma – EDTA, and plasma – heparin.

Interpolated concentration of native IL-13 receptor alpha 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 16 serum, 1 : 16 plasma – citrate, 1 : 16 plasma – EDTA, and 1 : 16 plasma – heparin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of human IL-13 receptor alpha 1 in HepG2 cell culture supernatant.

Interpolated concentration of native IL-13 receptor alpha 1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% for HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Example of human IL-13 receptor alpha 1 standard curve in Sample Diluent NS.

Example of human IL-13 receptor alpha 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of IL-13 receptor alpha 1 in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean IL-13 receptor alpha 1 concentration was determined to be 23.8 ng/mL with a range of 15.3 – 35.4 ng/mL

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.939 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-13 receptor alpha 1 SimpleStep ELISA® kit (ab303763) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-13 receptor alpha 1 protein in serum, plasma (cit), plasma (hep), plasma (EDTA) and cell culture supernatant. Quantitate Human IL-13 receptor alpha 1 with 5.939 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "109 - 117 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "84 - 100 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "93 - 110 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "82 - 113 %", "average": "= 100" }, { "sample": "Serum", "range": "90 - 113 %", "average": "= 105" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

See full target information IL13RA1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-13 receptor alpha 1 ELISA Kit

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

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Primary Antibodies

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Product promise