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AB46066

Human IL-13 Receptor alpha ELISA Kit

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Human IL-13 Receptor alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-13 Receptor alpha in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD213a2, IL13R, IL13RA2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Interleukin-13-binding protein

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Sandwich ELISA - Human IL-13 Receptor alpha ELISA Kit (AB46066)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-13 Receptor alpha ELISA Kit (AB46066)

Representative Standard Curve using ab46066

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 150 pg/mL

Range

156 - 5000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.

This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "2744 pg/mL", "standardDeviation": "90.1", "coefficientOfVariability": "3.28" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "725 pg/mL", "standardDeviation": "43.9", "coefficientOfVariability": "6.06" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 14, "mean": "3024 pg/mL", "standardDeviation": "93.67", "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 14, "mean": "1054 pg/mL", "standardDeviation": "29.69", "coefficientOfVariability": "2.82" } ]
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Recovery

[ { "sample": "Serum", "range": "79 - 100 %", "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46066-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46066-CMP03" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46066-CMP01" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46066-CMP02" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46066-CMP04" }, { "size":"2 x 1 Vial", "name":"IL-13 Receptor alpha Standard (Lyophilized)", "number":"AB46066-CMP09" }, { "size":"1 x 1 Unit", "name":"IL-13 Receptor alpha Microplate (12 x 8 well strips)", "number":"AB46066-CMP08" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-13 Receptor alpha", "number":"AB46066-CMP07" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46066-CMP10" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46066-CMP06" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46066-CMP05" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46066-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46066-CMP03", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-13 Receptor alpha", "number":"AB46066-CMP07", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-13 Receptor alpha Standard (Lyophilized)", "number":"AB46066-CMP09", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46066-CMP05", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46066-CMP10", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46066-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46066-CMP06", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46066-CMP04", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-13 Receptor alpha Microplate (12 x 8 well strips)", "number":"AB46066-CMP08", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46066-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed : 11861389, PubMed : 17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed : 11861389, PubMed : 17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed : 16327802).
See full target information IL13RA2
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websiteProtocolBooklet
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