Human IL-13 Receptor alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-13 Receptor alpha in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156 - 5000 pg/mL
- Assay time
- 3h 45m
- Sensitivity
- < 150 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed:11861389, PubMed:17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed:11861389, PubMed:17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed:16327802).
Alternative names
CD213a2, IL13R, IL13RA2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Interleukin-13-binding protein
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Human IL-13 Receptor alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-13 Receptor alpha in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156 - 5000 pg/mL
- Assay time
- 3h 45m
- Assay Platform
- Microplate
- Sensitivity
- < 150 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 14 | mean 3024 pg/mL | SD 93.67 | C.V. 3.1 |
Sample Sample B | n 14 | mean 1054 pg/mL | SD 29.69 | C.V. 2.82 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 8 | mean 2744 pg/mL | SD 90.1 | C.V. 3.28 |
Sample Sample B | n 8 | mean 725 pg/mL | SD 43.9 | C.V. 6.06 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 90 | Range 79 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.
A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.
This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.
Product promise
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Full details and terms and conditions can be found here:
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1 product image
Sandwich ELISA - Human IL-13 Receptor alpha ELISA Kit (ab46066)
Representative Standard Curve using ab46066
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Product protocols
- Visit the general protocols
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