Human IL-13R alpha 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-13R alpha 2 with a sensitivity of 96.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 281.3 - 18000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 96.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed:11861389, PubMed:17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed:11861389, PubMed:17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed:16327802).
Alternative names
CD213a2, IL13R, IL13RA2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Interleukin-13-binding protein
What's included?
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Human IL-13R alpha 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-13R alpha 2 with a sensitivity of 96.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 281.3 - 18000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 96.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 8 | mean - | SD - | C.V. 3.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 5.41 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 104 | Range 103 - 105 % |
Sample type Serum | Average % = 86 | Range 83 - 92 % |
Sample type EDTA Plasma | Average % = 91 | Range 90 - 94 % |
Sample type Cell culture extracts | Average % = 107 | Range 104 - 110 % |
Sample type Heparin Plasma | Average % = 88 | Range 86 - 93 % |
Sample type Citrate plasma | Average % = 80 | Range 78 - 82 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-13R alpha 2 ELISA kit (ab274400) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-13R alpha 2 protein in serum, plasma cell culture supernatant, and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-13R alpha 2 with 96.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-13 receptor alpha 2 is one of the receptors for IL-13, which is a T helper 2 molecule involved in immune response, inflammation, and wound healing. Studies have shown overexpression of IL-13R alpha 2 can confer invasive and metastatic ability in variety of cancer cells such as melanoma, ovarian, pancreatic, colorectal, and gliomas. Therefore, making IL-13R alpha 2 a viable candidate as a prognostic biomarker. This kit is developed to recognize and target only IL-13R alpha 2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human IL-13R alpha 2 ELISA Kit (ab274400)
Example of human IL-13R alpha 2 standard curve in Sample Diluent NS.
The IL-13R alpha 2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-13R alpha 2 ELISA Kit (ab274400)
Interpolated concentrations of native IL-13R alpha 2 in human cell culture supernatant sample.
The concentrations of IL-13R alpha 2 were measured in duplicates, interpolated from the IL-13R alpha 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: A375 supernatant neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13R alpha 2 concentration was determined to be 3,741 pg/mL in A375 supernatant.
Sandwich ELISA - Human IL-13R alpha 2 ELISA Kit (ab274400)
Interpolated concentrations of native IL-13R alpha 2 in human U-87MG and A375 samples based on a 400 μg/mL extract load.
The concentrations of IL-13R alpha 2 were measured in duplicate and interpolated from the IL-13R alpha 2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13R alpha 2 concentration was determined to be 8,147 pg/mL in U-87MG cell extract and 10,842 pg/mL in A375 cell extract.
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