AB264618

Human IL-15RA ELISA Kit

Recombinant
SimpleStep
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Human IL-15RA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-15RA with a sensitivity of 5.79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD215, Interleukin-15 receptor subunit alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, IL15RA

5 Images

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

sELISA

Lab

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Example of human IL-15RA standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Interpolated concentrations of native IL-15RA in human cell culture supernatant samples.

The concentrations of IL-15RA were measured in duplicates, interpolated from the IL-15RA standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated PBMCs 50% and stimulated PBMCs 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-15RA concentration was determined to be undetectable in unstimulated PBMCs and 90.8 pg/mL in stimulated PBMCs.

sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Interpolated concentrations of native IL-15RA in human serum and plasma.

The concentrations of IL-15RA were measured in duplicates, interpolated from the IL-15RA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-15RA concentration was determined to be 170.6 pg/mL in serum and 157.8 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Representative image of Human IL-15RA ELISA Kit (ab264618)

Components shown from left to right :

- Sample diluent NS

- 10X Human IL-15RA Capture Antibody

- 10X Human IL-15RA Detector Antibody

- Human IL-15RA Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Example of human IL-15RA standard curve in Sample Diluent NS + 1.5X Enhancer.

The IL-15RA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.79 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-15RA ELISA Kit (ab264618) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-15RA protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-15RA with 5.79 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-15 receptor subunit alpha is one of the three subunits that is part of its hematopoietin receptor family. IL-15RA contributes to IL-15 mediated effects such as cell survival, cell proliferation, and activation of T and B lymphocytes. This kit recognizes extracellular fragment of IL-15RA, which is solubilized and binds to IL-15 with high affinity. IL-15 does not interfere in ab264618.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 96 %", "average": "= 90" }, { "sample": "Serum", "range": "107 - 121 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "96 - 121 %", "average": "= 110" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab264618-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB264618-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264618-CMP11" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Capture Antibody", "number":"AB264618-CMP08" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264618-CMP01" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264618-CMP06" }, { "size":"2 x 1 Vial", "name":"Human IL-15RA Lyophilized Recombinant Protein", "number":"AB264618-CMP07" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Detector Antibody", "number":"AB264618-CMP09" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264618-CMP10" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB264618-CMP04" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264618-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB264618-CMP12" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264618-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human IL-15RA Lyophilized Recombinant Protein", "number":"AB264618-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264618-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Capture Antibody", "number":"AB264618-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264618-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264618-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264618-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264618-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264618-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264618-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Detector Antibody", "number":"AB264618-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264618-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

High-affinity receptor for interleukin-15 (PubMed : 8530383). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (By similarity). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (PubMed : 15123770). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (PubMed : 15123770). Expression of different isoforms may alter or interfere with signal transduction (PubMed : 10480910).. Isoform 5. Does not bind IL15.. Isoform 6. Does not bind IL15.. Isoform 7. Does not bind IL15.. Isoform 8. Does not bind IL15.

See full target information IL15RA

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-15RA ELISA Kit

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

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Product promise