AB324411

Human IL-16 ELISA Kit

SimpleStep
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Human IL-16 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-16 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Pro-interleukin-16, IL16

6 Images

Sandwich ELISA - Human IL-16 ELISA Kit (AB324411)

sELISA

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Sandwich ELISA - Human IL-16 ELISA Kit (AB324411)

Standard curve comparison between the original Human IL-16 SimpleStep ELISA (ab193763) and current Human IL-16 SimpleStep ELISA (ab324411).

sELISA

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Sandwich ELISA - Human IL-16 ELISA Kit (AB324411)

Example of human IL-16 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

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Sandwich ELISA - Human IL-16 ELISA Kit (AB324411)

Interpolated concentrations of IL-16 in serum of ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean IL-16 concentration was determined to be 497.7 pg/mL with a range of 177.9 – 826.4 pg/mL.

sELISA

Lab

Sandwich ELISA - Human IL-16 ELISA Kit (AB324411)

Interpolated concentration of native IL-16 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human IL-16 ELISA Kit (AB324411)

Standard curve comparison between Human IL-16 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Lab

Sandwich ELISA - Human IL-16 ELISA Kit (AB324411)

Cell supernatants of 48-hour cultures of unstimulated and 1 g/mL PHA-M stimulated PBMC were measured in duplicates. Undiluted samples are unstimulated PBMC supernatant 25%, and stimulated PBMC supernatant 8%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No signal was observed in neat cell culture media (RPMI 1640 containing 10% fetal bovine serum).

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.899 pg/mL

Range

4.688 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-16 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-16 with a sensitivity of 1.899pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

- Different format for different needs: 10 x 96 plates for bulk orders.

- Design your own immunoassay: we also offer the conjugation-ready antibody pair.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.2" } ]

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Recovery

[ { "sample": "Serum", "range": "82 - 98 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "105 - 125 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "97 - 105 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "106 - 119 %", "average": "= 113" }, { "sample": "Cell culture supernatant", "range": "96 - 107 %", "average": "= 100" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.. Isoform 1. May act as a scaffolding protein that anchors ion channels in the membrane.. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

See full target information IL16

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websiteProtocolBooklet

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Product promise

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