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Human IL-17 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-17 in Human in Plasma, Cell culture supernatant samples.

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Sandwich ELISA - Human IL-17 ELISA Kit (AB100556), expandable thumbnail
  • Sandwich ELISA - Human IL-17 ELISA Kit (AB100556), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.63 - 1000 pg/mL

Sensitivity

< 10 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed:24120361). Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation (PubMed:17911633, PubMed:18684971, PubMed:19825828, PubMed:21350122, PubMed:24120361, PubMed:8676080). Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (By similarity). In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines (By similarity). In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells (By similarity). Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance (By similarity). Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation (By similarity). Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection (PubMed:21350122). Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis (By similarity). As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release (By similarity). In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (By similarity). Involved in antiviral host defense through various mechanisms (By similarity). Enhances immunity against West Nile virus by promoting T cell cytotoxicity (By similarity). May play a beneficial role in influenza A virus (H5N1) infection by enhancing B cell recruitment and immune response in the lung (By similarity). Contributes to influenza A virus (H1N1) clearance by driving the differentiation of B-1a B cells, providing for production of virus-specific IgM antibodies at first line of host defense (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
120X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B
1 x 15 mL
Assay Diluent A
1 x 30 mL
Biotinylated anti-Human IL-17
2 x 1 Vial
IL-17 Microplate (12 x 8 wells)
1 x 1 Unit
Recombinant Human IL-17 Standard (lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human IL-17 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-17 in Human in Plasma, Cell culture supernatant samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

15.63 - 1000 pg/mL

Assay Platform

Microplate

Sensitivity

< 10 pg/mL

General recovery

93%

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 93.65

Range

84 - 103 %

Sample type

Plasma

Average %

= 92.24

Range

82 - 104 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's IL-17 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17 in plasma and cell culture supernatants.

This assay employs an antibody specific for Human IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human IL-17 in normal serum with ab100556, only in serum spiked with Human IL-17.

Optimization may be required with urine samples

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human IL-17 ELISA Kit (ab100556), expandable thumbnail

    Sandwich ELISA - Human IL-17 ELISA Kit (ab100556)

    Representative standard curve using ab100556

  • Sandwich ELISA - Human IL-17 ELISA Kit (ab100556), expandable thumbnail

    Sandwich ELISA - Human IL-17 ELISA Kit (ab100556)

    Representative standard curve using ab100556

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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