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AB279410

Human IL-17C ELISA Kit

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Human IL-17C ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-17C with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

UNQ561/PRO1122, IL17C, Interleukin-17C, IL-17C, Cytokine CX2

2 Images
Sandwich ELISA - Human IL-17C ELISA Kit (AB279410)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17C ELISA Kit (AB279410)

Interpolated concentrations of native IL-17C in human HCT 116 cell culture supernatant sample treated with 15 ng/mL Hu IL-1β for 24 hours.

The concentrations of IL-17C were measured in duplicates, interpolated from the IL-17C standard curves and corrected for sample dilution. Undiluted samples are as follows : treated HCT 116 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17C concentration was determined to be 93.5 pg/mL in neat treated supernatant. There was no detectable signal in mock treated HCT 116 cell supernatant.

Sandwich ELISA - Human IL-17C ELISA Kit (AB279410)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17C ELISA Kit (AB279410)

Example of human IL-17C standard curve in Sample Diluent NS.

The IL-17C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.2 pg/mL

Range

9.38 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-17C ELISA kit (ab279410) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-17C protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17C with 1.2 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-17C, also known as CX2, is a secreted cytokine of IL-17 family. IL-17C binds to a heterodimer formed by IL-17RA and IL-17RE. IL-17C plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. It stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. IL-17C is up-regulated by bacterial stimuli, including by lipopolysaccharides in gut epithelial cell lines, and by various proinflammatory cytokines, including TNF, IL-1B and IL-17F. IL-17C acts synergistically with IL-22 in inducing the expression of antibacterial peptides, and with TNF and IL-1B in inducing DEFB2 from keratinocytes. Depending on the type of insult, IL-17C may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. It plays an important role in the pathogenesis of inflammatory arthritis.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 127 %", "average": "= 111" }, { "sample": "Serum", "range": "101 - 104 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "109 - 117 %", "average": "= 114" }, { "sample": "Heparin Plasma", "range": "96 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "104 - 116 %", "average": "= 109" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279410-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB279410-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279410-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB279410-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279410-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279410-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB279410-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB279410-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB279410-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-17C Lyophilized Recombinant Protein", "number":"AB279410-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17C Capture Antibody", "number":"AB279410-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17C Detector Antibody", "number":"AB279410-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and pro-inflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. Synergy is also observed with TNF and IL1B in inducing DEFB2 from keratinocytes. Depending on the type of insult, may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases.
See full target information IL17C
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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