AB327298
Human IL-17C ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Human IL-17C ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IL-17C with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.74 - 2000 pg/ml with a sensitivity of 0.6 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 2.74 - 2000 pg/ml with a sensitivity of 0.6 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
3 Images
- sELISA
Supplier Data
Sandwich ELISA - Human IL-17C ELISA Kit, Chemiluminescent (AB327298)
Interpolated concentrations of native IL-17C in human HCT 116 cell culture supernatant sample treated with 15 ng/mL Hu IL-1β for 24 hours.
The concentrations of IL-17C were measured in duplicates interpolated from the IL-17C standard curves and corrected for sample dilution. Undiluted samples are as follows : treated HCT 116 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IL-17C concentration was determined to be 93.5 pg/mL in neat treated supernatant. There was no detectable signal in mock treated HCT 116 cell supernatant.
- sELISA
Lab
Sandwich ELISA - Human IL-17C ELISA Kit, Chemiluminescent (AB327298)
Standard curve comparison between the Chemiluminescent Human IL-17C SimpleStep ELISA and colorimetric Human IL-17C SimpleStep ELISA (ab279410) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human IL-17C ELISA Kit, Chemiluminescent (AB327298)
Dynamic range comparison between the Chemiluminescent Human IL-17C SimpleStep ELISA, colorimetric Human IL-17C SimpleStep ELISA (ab279410), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human IL-17C ELISA Kit, Chemiluminescent (ab327298) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-17C in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 0.6 pg/mL.
How the assay works
Human IL-17C ELISA Kit, Chemiluminescent (ab327298) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human IL-17C (ab327298) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL17C antibody [EPR23144-57] - BSA and Aside free (Capture) ab281530
- Anti-IL17C antibody [EPR23144-101] - BSA and Aside free (Detector) ab281554
How the assay works
Human IL-17C ELISA Kit, Chemiluminescent (ab327298) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human IL-17C (ab327298) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL17C antibody [EPR23144-57] - BSA and Aside free (Capture) ab281530
- Anti-IL17C antibody [EPR23144-101] - BSA and Aside free (Detector) ab281554
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
Recovery
[ { "sample": "Cell culture supernatant", "range": "103 - 127 %", "average": "= 111" }, { "sample": "Serum", "range": "101 - 104 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "109 - 117 %", "average": "= 114" }, { "sample": "Heparin Plasma", "range": "96 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "104 - 116 %", "average": "= 109" } ]
What's included?
{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab327298-10x96Tests", "assetComponentDetails": [ { "size":"10 x 600 µL", "name":"10X Human IL-17C Detector Antibody", "number":"AB327298-CMP02" }, { "size":"10 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB327298-CMP05" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent B", "number":"AB327298-CMP09" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327298-CMP06" }, { "size":"10 x 600 µL", "name":"10X Human IL-17C Capture Antibody", "number":"AB327298-CMP01" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB327298-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327298-CMP10" }, { "size":"1 x 50 mL", "name":"ChemiHRP Reagent A", "number":"AB327298-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327298-CMP07" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327298-CMP04" }, { "size":"10 x 1 Vial", "name":"Human IL-17C Lyophilized Recombinant Protein", "number":"AB327298-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab327298-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB327298-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB327298-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB327298-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB327298-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-17C Lyophilized Recombinant Protein", "number":"AB327298-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17C Detector Antibody", "number":"AB327298-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB327298-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB327298-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB327298-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB327298-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17C Capture Antibody", "number":"AB327298-CMP01", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and pro-inflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. Synergy is also observed with TNF and IL1B in inducing DEFB2 from keratinocytes. Depending on the type of insult, may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. Enhanced IL17C/IL17RE signaling may also lead to greater susceptibility to autoimmune diseases.
Alternative Names
UNQ561/PRO1122, IL17C, Interleukin-17C, IL-17C, Cytokine CX2
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com