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AB277723

Human IL-17D ELISA Kit

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Human IL-17D ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-17D with a sensitivity of 11 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

UNQ3096/PRO21175, IL17D, Interleukin-17D, IL-17D, Interleukin-27, IL-27

2 Images
Sandwich ELISA - Human IL-17D ELISA Kit (AB277723)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17D ELISA Kit (AB277723)

Interpolated concentrations of spike IL-17D in human serum, plasma and cell culture supernatant samples.

The concentrations of IL-17D were measured in duplicates, interpolated from the IL-17D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, plasma (heparin) 100% and cell culture media (RPMI 1640 containing 10% fetal bovine serum) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-17D ELISA Kit (AB277723)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17D ELISA Kit (AB277723)

Example of human IL-17D standard curve in Sample Diluent 50BP.

The IL-17D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-17D ELISA kit (ab277723) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-17D protein in human serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17D with 11 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-17D is a secreted cytokine of IL-17 protein family. IL-17D induces expression of IL6, CXCL8/IL-8, and CSF2/GM-CSF from endothelial cells.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 87 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "83 - 88 %", "average": "= 85" }, { "sample": "Cell culture media", "range": "101 - 107 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "89 - 95 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "96 - 106 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277723-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277723-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB277723-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277723-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-17D Lyophilized Recombinant Protein", "number":"AB277723-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277723-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17D Capture Antibody", "number":"AB277723-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BP", "number":"AB277723-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277723-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277723-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277723-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17D Detector Antibody", "number":"AB277723-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Induces expression of IL6, CXCL8/IL8, and CSF2/GM-CSF from endothelial cells.
See full target information IL17D
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com