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AB254518

Human IL-17RB ELISA Kit

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Human IL-17RB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-17RB with a sensitivity of 64 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CRL4, EVI27, IL17BR, UNQ2501/PRO19612, IL17RB, Interleukin-17 receptor B, IL-17 receptor B, IL-17RB, Cytokine receptor-like 4, IL-17 receptor homolog 1, Interleukin-17B receptor, IL-17Rh1, IL17Rh1, IL-17B receptor

2 Images
Sandwich ELISA - Human IL-17RB ELISA Kit (AB254518)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17RB ELISA Kit (AB254518)

Interpolated concentrations of spike IL-17RB in human cell culture supernatant samples.

The concentrations of IL-17RB were measured in duplicates, interpolated from the IL-17RB standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-17RB ELISA Kit (AB254518)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-17RB ELISA Kit (AB254518)

Example of human IL-17RB standard curve in Sample Diluent NS.

The IL-17RB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 64 pg/mL

Range

137.5 - 8800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-17RB ELISA Kit (ab254518) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17RB protein in cell culture media and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17RB with 64 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-17RB is a plasma membrane receptor for the proinflammatory cytokines IL-17B and IL-17E. IL-17RB may play a role in controlling the growth and/or differentiation of hematopoietic cells. An alternative spliced secreted variant of IL-17RB has also been identified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 95" }, { "sample": "Cell culture media", "range": null, "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254518-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254518-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254518-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254518-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254518-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB254518-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB254518-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-17RB Lyophilized Recombinant Protein", "number":"AB254518-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17RB Capture Antibody", "number":"AB254518-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-17RB Detector Antibody", "number":"AB254518-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254518-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for the pro-inflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells.
See full target information IL17RB
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websiteProtocolBooklet
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