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AB325627

Human IL-18 ELISA Kit, Chemiluminescent

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Human IL-18 ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IL-18 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 3.66 - 8000 pg/ml with a sensitivity of 2.4 pg/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma

6 Images
Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)
  • sELISA

Lab

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)
  • sELISA

Lab

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)

Standard curve comparison between the Chemiluminescent Human IL-18 SimpleStep ELISA and colorimetric Human IL-18 SimpleStep ELISA (ab215539) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)
  • sELISA

Lab

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)

Standard curve comparison between the Chemiluminescent Human IL-18 SimpleStep ELISA and colorimetric Human IL-18 SimpleStep ELISA (ab215539) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)
  • sELISA

Lab

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)

Dynamic range comparison between the Chemiluminescent Human IL-18 SimpleStep ELISA, colorimetric Human IL-18 SimpleStep ELISA (ab215539), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and range.

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)
  • sELISA

Lab

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)

Interpolated concentrations of native IL-18 in human HeLa, HL-60, and A431 cell extract samples based on a 800 μg/ml extract load. The concentrations of IL-18 were measured in duplicate and interpolated from the IL-18 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 1,099 pg/ml in HeLa cell extract, 456 pg/ml in HL-60 cell extract and 567 pg/ml in A431 cell extract.

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)
  • sELISA

Lab

Sandwich ELISA - Human IL-18 ELISA Kit, Chemiluminescent (AB325627)

Serum from nine individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 423 pg/mL with a range of undetectable – 2,852 pg/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine, Cell Extract

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.4 pg/mL

Range

3.66 - 8000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human IL-18 ELISA Kit, Chemiluminescent (ab325627) is a rapid single-wash sandwich 55 min ELISA to measure Human IL-18 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Urine, Cell Extract with a sensitivity of 2.4 pg/mL.

How the assay works

Human IL-18 ELISA Kit, Chemiluminescent (ab325627) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-18 (ab325627) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (Capture) ab242590
- Anti-IL-18 antibody [EPR19956-19] - BSA and Azide free (Detector) ab242856
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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.7" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 14.2" } ]
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Recovery

[ { "sample": "Serum", "range": "80 - 91 %", "average": "= 84" }, { "sample": "Citrate plasma", "range": "80 - 84 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "82 - 91 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "91 - 97 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "89 - 104 %", "average": "= 96" }, { "sample": "Urine", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Cell Lysate", "range": "93 - 101 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed : 14528293, PubMed : 25500532, PubMed : 37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed : 10653850). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33883744).
See full target information IL18
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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