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AB113325

Human IL-18 R beta ELISA Kit

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Human IL-18 R beta ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-18 R beta in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human IL-18 R beta ELISA Kit (AB113325)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 R beta ELISA Kit (AB113325)

Representative Standard Curve using ab113325

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 12 pg/mL

Range

12.3 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-18 R beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "68 - 85 %", "average": "= 75.78" }, { "sample": "Serum", "range": "71 - 89 %", "average": "= 80.9" }, { "sample": "Plasma", "range": "67 - 83 %", "average": "= 74.98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113325-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113325-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113325-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-18 R beta Microplate", "number":"AB113325-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-18 R beta Standard (lyophilized)", "number":"AB113325-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113325-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB113325-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113325-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-18 R beta", "number":"AB113325-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB113325-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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websiteProtocolBooklet
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Product promise

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