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AB100559

Human IL-18BP ELISA Kit

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(3 Publications)

Human IL-18BP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-18BP in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-18-binding protein, IL-18BP, Tadekinig-alfa, IL18BP

2 Images
Sandwich ELISA - Human IL-18BP ELISA Kit (AB100559)
  • sELISA

Unknown

Sandwich ELISA - Human IL-18BP ELISA Kit (AB100559)

Representative standard curve using ab100559

Sandwich ELISA - Human IL-18BP ELISA Kit (AB100559)
  • sELISA

Unknown

Sandwich ELISA - Human IL-18BP ELISA Kit (AB100559)

Representative standard curve using ab100559

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

24.69 - 18000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-18BP (Interleukin-18 Binding Protein) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18BP in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human IL-18BP coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 121 %", "average": "= 106.7" }, { "sample": "Serum", "range": "74 - 97 %", "average": "= 83.5" }, { "sample": "Plasma", "range": "72 - 96 %", "average": "= 82.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100559-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100559-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100559-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-18BP", "number":"AB100559-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100559-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-18BP Standard (lyophilized)", "number":"AB100559-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-18BP Microplate (12 x 8 wells)", "number":"AB100559-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100559-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100559-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB100559-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
See full target information Interleukin-18-binding protein
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology reports 46:1239-1246 PubMed30632070

2019

Potential role of serum fetuin-A in relation with pro-inflammatory, chemokine and adhesion molecules in diabetic kidney disease: a case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Shaik Sarfaraz Nawaz,Salini Scaria Joy,Yousuf Al Farsi,Teena P George,Khalid Siddiqui

Journal of molecular endocrinology 54:289-303 PubMed25878060

2015

Bisphenol A effects on gene expression in adipocytes from children: association with metabolic disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Ciro Menale,Maria Teresa Piccolo,Grazia Cirillo,Raffaele A Calogero,Alfonso Papparella,Luigi Mita,Emanuele Miraglia Del Giudice,Nadia Diano,Stefania Crispi,Damiano Gustavo Mita

EMBO molecular medicine 6:821-34 PubMed24778454

2014

Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Uzan,Sandrine Poglio,Bastien Gerby,Ching-Lien Wu,Julia Gross,Florence Armstrong,Julien Calvo,Xavier Cahu,Caroline Deswarte,Florent Dumont,Diana Passaro,Corinne Besnard-Guérin,Thierry Leblanc,André Baruchel,Judith Landman-Parker,Paola Ballerini,Véronique Baud,Jacques Ghysdael,Frédéric Baleydier,Francoise Porteu,Francoise Pflumio
View all publications
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websiteProtocolBooklet
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Product promise

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