Skip to main content

Human IL-18BP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-18BP in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877), expandable thumbnail
  • Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877), expandable thumbnail
  • Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877), expandable thumbnail
  • Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

156 - 10000 pg/mL

Assay time

1h 30m

Sensitivity

= 1.9 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

1 product for Alternative Version

Target data

Function

Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.

Alternative names

What's included?

1 x 96 Tests
Components
10X Human IL-18BP Capture Antibody
1 x 600 µL
10X Human IL-18BP Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Human IL-18BP Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human IL-18BP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-18BP in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

156 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 1.9 pg/mL

Precision

Intra assay

Sample

Serum

n

5

mean

-

SD

-

C.V.

3.5

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

3.3

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 115

Range

103 - 122 %

Sample type

EDTA Plasma

Average %

= 86

Range

78 - 90 %

Sample type

Urine

Average %

= 94

Range

87 - 102 %

Sample type

Heparin Plasma

Average %

= 90

Range

74 - 102 %

Sample type

Citrate plasma

Average %

= 86

Range

80 - 90 %

Sample type

Cell culture media

Average %

= 103

Range

99 - 108 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human IL-18BP ELISA Kit (ab224877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-18BP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-18BP with 1.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-18 binding protein is a secreted glycoprotein that functions as an IL-18 antagonist by preventing the interaction of IL-18 with IL-18 Receptor alpha. Isoform A of IL-18 Binding Protein binds to IL-18 and inhibits its activity. IL-18 Binding Protein functions as an inhibitor of the early TH1 cytokine response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877), expandable thumbnail

    Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877)

    Example of HumanIL-18 Binding Protein standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877), expandable thumbnail

    Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877)

    Interpolated concentrations of native IL-18 Binding Protein in Human serum and plasma samples.

    The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL in neat serum, 2682 pg/mL in neat plasma (citrate), 3360 pg/ml in neat plasma (heparin), 2957 pg/mL in neat plasma (EDTA), and 11,400 pg/ml in neat urine.

  • Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877), expandable thumbnail

    Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877)

    Interpolated concentrations of spiked IL-18 Binding Protein in Human cell culture supernatant and media samples.

    The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: THP-1 supernatant 100% and RPMI 1640 Media (containing 10% fetal bovine serum) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877), expandable thumbnail

    Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877)

    Serum from ten individual healthy Human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL with a range of 3081 – 6405 pg/mL.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com