Human IL-18BP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-18BP in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
156 - 10000 pg/mL
1h 30m
= 1.9 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
Interleukin-18-binding protein, IL-18BP, Tadekinig-alfa, IL18BP
Human IL-18BP ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-18BP in Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
156 - 10000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 1.9 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 3.5 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.3 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 115 | Range 103 - 122 % |
Sample type EDTA Plasma | Average % = 86 | Range 78 - 90 % |
Sample type Urine | Average % = 94 | Range 87 - 102 % |
Sample type Heparin Plasma | Average % = 90 | Range 74 - 102 % |
Sample type Citrate plasma | Average % = 86 | Range 80 - 90 % |
Sample type Cell culture media | Average % = 103 | Range 99 - 108 % |
Blue Ice
+4°C
+4°C
+4°C
Human IL-18BP ELISA Kit (ab224877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-18BP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-18BP with 1.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-18 binding protein is a secreted glycoprotein that functions as an IL-18 antagonist by preventing the interaction of IL-18 with IL-18 Receptor alpha. Isoform A of IL-18 Binding Protein binds to IL-18 and inhibits its activity. IL-18 Binding Protein functions as an inhibitor of the early TH1 cytokine response.
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Example of HumanIL-18 Binding Protein standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native IL-18 Binding Protein in Human serum and plasma samples.
The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL in neat serum, 2682 pg/mL in neat plasma (citrate), 3360 pg/ml in neat plasma (heparin), 2957 pg/mL in neat plasma (EDTA), and 11,400 pg/ml in neat urine.
Interpolated concentrations of spiked IL-18 Binding Protein in Human cell culture supernatant and media samples.
The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: THP-1 supernatant 100% and RPMI 1640 Media (containing 10% fetal bovine serum) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Serum from ten individual healthy Human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL with a range of 3081 – 6405 pg/mL.
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