AB224877

Human IL-18BP ELISA Kit

Recombinant
SimpleStep
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Human IL-18BP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-18BP with a sensitivity of 1.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-18-binding protein, IL-18BP, Tadekinig-alfa, IL18BP

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Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

sELISA

Supplier Data

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Interpolated concentrations of native IL-18 Binding Protein in Human serum and plasma samples.

The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL in neat serum, 2682 pg/mL in neat plasma (citrate), 3360 pg/ml in neat plasma (heparin), 2957 pg/mL in neat plasma (EDTA), and 11,400 pg/ml in neat urine.

sELISA

Supplier Data

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Interpolated concentrations of spiked IL-18 Binding Protein in Human cell culture supernatant and media samples.

sELISA

Supplier Data

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Serum from ten individual healthy Human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL with a range of 3081 – 6405 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Example of HumanIL-18 Binding Protein standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.9 pg/mL

Range

156 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-18BP ELISA Kit (ab224877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-18BP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-18BP with 1.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-18 binding protein is a secreted glycoprotein that functions as an IL-18 antagonist by preventing the interaction of IL-18 with IL-18 Receptor alpha. Isoform A of IL-18 Binding Protein binds to IL-18 and inhibits its activity. IL-18 Binding Protein functions as an inhibitor of the early TH1 cytokine response.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Serum", "range": "103 - 122 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "78 - 90 %", "average": "= 86" }, { "sample": "Urine", "range": "87 - 102 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "74 - 102 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "80 - 90 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "99 - 108 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.

See full target information Interleukin-18-binding protein

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-18BP ELISA Kit

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise