AB231922

Human IL-19 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Human IL-19 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-19 with a sensitivity of 0.648 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ZMDA1, IL19, Interleukin-19, IL-19, Melanoma differentiation-associated protein-like protein, NG.1

5 Images

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

sELISA

Supplier Data

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Interpolated concentrations of spiked IL-19 in human serum and plasma samples.

The concentrations of IL-19 were measured in duplicate, interpolated from the IL-19 standard curves.

sELISA

Supplier Data

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Interpolated concentrations of native IL-19 in human urine (25%), samples.

The concentrations of IL-19 was measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 91.95 pg/mL in urine.

sELISA

Unknown

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Human IL-19 Competitor standard curve comparison.

Standard curve comparison between Human IL-19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Interpolated concentrations of native IL-19 in human milk (0.5%), saliva (10%), and PBMC supernatant (50%) samples.

The concentrations of IL-19 were measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 37.14 ng/mL in milk, 91.95 pg/mL in urine, 779.30 pg/mL in saliva, and 1008.97 pg/mL in PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Example of human IL-19 standard curve in Sample Diluent NS.

The IL-19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.648 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-19 ELISA Kit (ab231922) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-19 protein in cit plasma, edta plasma, hep plasma, milk, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-19 with 0.648 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Milk", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Milk", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 113 %", "average": "= 109" }, { "sample": "Milk", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Serum", "range": "86 - 90 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "87 - 88 %", "average": "= 87" }, { "sample": "Saliva", "range": "105 - 108 %", "average": "= 106" }, { "sample": "Urine", "range": "81 - 86 %", "average": "= 83" }, { "sample": "Heparin Plasma", "range": "88 - 92 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "86 - 88 %", "average": "= 86" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231922-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231922-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231922-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231922-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231922-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231922-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231922-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-19 Capture Antibody", "number":"AB231922-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-19 Lyophilized Recombinant Protein", "number":"AB231922-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB231922-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-19 Detector Antibody", "number":"AB231922-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that functions as an anti-inflammatory and proangiogenic factor (PubMed : 34932373). Polarizes adaptive immunity to an anti-inflammatory phenotype through induction of T-helper 2 responses by both down-regulation of IFN-gamma and up-regulation of IL4 and IL13 (PubMed : 16365913). Produced by osteocytes, stimulates granulopoiesis and neutrophil formation (By similarity). Exerts its biological effect through a receptor complex consisting of a heterodimer of IL20RA and IL20RB (PubMed : 12351624). In turn, activates the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) pathway, and importantly, STAT3 (PubMed : 11564763).

See full target information IL19

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 132: PubMed36006737

2022

IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfei He,Wenqian Luo,Yingjie Liu,Yuan Wang,Chengxin Ma,Qiuyao Wu,Pu Tian,Dasa He,Zhenchang Jia,Xianzhe Lv,Yu-Shui Ma,Haitang Yang,Ke Xu,Xue Zhang,Yansen Xiao,Peiyuan Zhang,Yajun Liang,Da Fu,Feng Yao,Guohong Hu

Scientific reports 12:16019 PubMed36163397

2022

Upregulation of interleukin-19 in saliva of patients with COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Shirin Hafezi,Swati Goel,Hawra Ali Hussain Alsayed,Abdul Wahid Ansari,Bassam Mahboub,Saleh Al-Muhsen,Mohamad-Hani Temsah,Qutayba Hamid,Rabih Halwani

ERJ open research 7: PubMed34322544

2021

Upregulation of interleukin-19 in severe asthma: a potential saliva biomarker for asthma severity.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Swati Goel,Bassam Mahboub,Abdul Wahid Ansari,Mohamad-Hani Temsah,Adel M Zakri,Elaref Ratemi,Rifat Hamoudi,Qutayba Hamid,Rabih Halwani

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-19 ELISA Kit

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Sandwich ELISA - Human IL-19 ELISA Kit (AB231922)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

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Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Product promise