Human IL-19 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-19 with a sensitivity of 0.648 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Publications
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- The Journal of clinical investigation 132:2022IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfei He,Wenqian Luo,Yingjie Liu,Yuan Wang,Chengxin Ma,Qiuyao Wu,Pu Tian,Dasa He,Zhenchang Jia,Xianzhe Lv,Yu-Shui Ma,Haitang Yang,Ke Xu,Xue Zhang,Yansen Xiao,Peiyuan Zhang,Yajun Liang,Da Fu,Feng Yao,Guohong HuPubMed 36006737
- Scientific reports 12:160192022Upregulation of interleukin-19 in saliva of patients with COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Shirin Hafezi,Swati Goel,Hawra Ali Hussain Alsayed,Abdul Wahid Ansari,Bassam Mahboub,Saleh Al-Muhsen,Mohamad-Hani Temsah,Qutayba Hamid,Rabih HalwaniPubMed 36163397
- ERJ open research 7:2021Upregulation of interleukin-19 in severe asthma: a potential saliva biomarker for asthma severity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFatemeh Saheb Sharif-Askari,Narjes Saheb Sharif-Askari,Swati Goel,Bassam Mahboub,Abdul Wahid Ansari,Mohamad-Hani Temsah,Adel M Zakri,Elaref Ratemi,Rifat Hamoudi,Qutayba Hamid,Rabih HalwaniPubMed 34322544
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 7.81 - 500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.648 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that functions as an anti-inflammatory and proangiogenic factor (PubMed:34932373). Polarizes adaptive immunity to an anti-inflammatory phenotype through induction of T-helper 2 responses by both down-regulation of IFN-gamma and up-regulation of IL4 and IL13 (PubMed:16365913). Produced by osteocytes, stimulates granulopoiesis and neutrophil formation (By similarity). Exerts its biological effect through a receptor complex consisting of a heterodimer of IL20RA and IL20RB (PubMed:12351624). In turn, activates the Janus kinase (JAK) and signal transducer and activator of transcription (STAT) pathway, and importantly, STAT3 (PubMed:11564763).
Alternative names
ZMDA1, IL19, Interleukin-19, IL-19, Melanoma differentiation-associated protein-like protein, NG.1
What's included?
Recommended products
Human IL-19 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-19 with a sensitivity of 0.648 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 7.81 - 500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.648 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Milk | n 8 | mean - | SD - | C.V. 2.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Milk | n 3 | mean - | SD - | C.V. 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 109 | Range 103 - 113 % |
Sample type Milk | Average % = 100 | Range 98 - 103 % |
Sample type Serum | Average % = 89 | Range 86 - 90 % |
Sample type EDTA Plasma | Average % = 87 | Range 87 - 88 % |
Sample type Saliva | Average % = 106 | Range 105 - 108 % |
Sample type Urine | Average % = 83 | Range 81 - 86 % |
Sample type Heparin Plasma | Average % = 89 | Range 88 - 92 % |
Sample type Citrate plasma | Average % = 86 | Range 86 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-19 ELISA Kit (ab231922) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-19 protein in cit plasma, edta plasma, hep plasma, milk, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-19 with 0.648 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Sandwich ELISA - Human IL-19 ELISA Kit (ab231922)
Human IL-19 Competitor standard curve comparison.
Standard curve comparison between Human IL-19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human IL-19 ELISA Kit (ab231922)
Example of human IL-19 standard curve in Sample Diluent NS.
The IL-19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-19 ELISA Kit (ab231922)
Interpolated concentrations of spiked IL-19 in human serum and plasma samples.
The concentrations of IL-19 were measured in duplicate, interpolated from the IL-19 standard curves.
Sandwich ELISA - Human IL-19 ELISA Kit (ab231922)
Interpolated concentrations of native IL-19 in human milk (0.5%), saliva (10%), and PBMC supernatant (50%) samples.
The concentrations of IL-19 were measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 37.14 ng/mL in milk, 91.95 pg/mL in urine, 779.30 pg/mL in saliva, and 1008.97 pg/mL in PBMC supernatant.
Sandwich ELISA - Human IL-19 ELISA Kit (ab231922)
Interpolated concentrations of native IL-19 in human urine (25%), samples.
The concentrations of IL-19 was measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 91.95 pg/mL in urine.
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