Human IL-1ra ELISA Kit ab211650 is a single-wash 90-min SimpleStep ELISA used to quantify Human IL-1ra with a sensitivity of 1.5 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human IL-1ra sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
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Publications
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- iScience 26:1068732023induces trained immunity in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiquel de Homdedeu,Lidia Sanchez-Moral,Concepció Violán,Neus Ràfols,Dan Ouchi,Berta Martín,Miguel A Peinado,Alhelí Rodríguez-Cortés,Marta Arch-Sisquella,Daniel Perez-Zsolt,Jordana Muñoz-Basagoiti,Nuria Izquierdo-Useros,Betlem Salvador,Joan Matllo,Sergi López-Serrano,Joaquim Segalés,Cristina Vilaplana,Pere Torán-Monserrat,Rosa Morros,Ramon Monfà,Maria-Rosa Sarrias,Pere-Joan CardonaPubMed 37250788
- Cytokine 153:1558682022Clinical monitored in subjects metabolically healthy and unhealthy before and during a SARS-CoV-2 infection- A cross-sectional study in Mexican population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel Treviño,Steffany Cortezano-Esteban,Hugo Hernández-Fragoso,Alfonso Díaz,Rubén Vázquez-Roque,Victor Enrique Sarmiento-Ortega,Diana Moroni-González,Rosana Pelayo,Eduardo BrambilaPubMed 35358903
- Journal of neuroinflammation 18:1892021Interleukin-1 blockade attenuates white matter inflammation and oligodendrocyte loss after progressive systemic lipopolysaccharide exposure in near-term fetal sheep.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharmony B Kelly,Vanesa Stojanovska,Valerie A Zahra,Alison Moxham,Suzanne L Miller,Timothy J M Moss,Stuart B Hooper,Marcel F Nold,Claudia A Nold-Petry,Justin M Dean,Laura Bennet,Graeme R Polglase,Alistair J Gunn,Robert GalinskyPubMed 34465372
- Rheumatology (Oxford, England) :2020New variant in the IL1RN-gene (DIRA) associated with late-onset, CRMO-like presentation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJasmin B Kuemmerle-Deschner et. al.PubMed 32259833
- Cellular immunology 327:77-822018Interleukin-1β, interleukin-1 receptor antagonist, interleukin-6, interleukin-10, and tumor necrosis factor-α levels in CSF and serum in relation to the clinical diversity of Parkinson's disease.
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Milk, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
7.81 - 500 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.5 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Alternative names
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, IL1RN, IL1F3, IL1RA
What's included?
Recommended products
Human IL-1ra ELISA Kit ab211650 is a single-wash 90-min SimpleStep ELISA used to quantify Human IL-1ra with a sensitivity of 1.5 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human IL-1ra sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Milk, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
7.81 - 500 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample EDTA Plasma | n 5 | mean - | SD - | C.V. 3.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample EDTA Plasma | n 3 | mean - | SD - | C.V. 4.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 98 | Range 97 - 99 % |
Sample type Milk | Average % = 104 | Range 98 - 110 % |
Sample type Serum | Average % = 106 | Range 82 - 119 % |
Sample type Urine | Average % = 102 | Range 100 - 106 % |
Sample type Saliva | Average % = 102 | Range 96 - 112 % |
Sample type Citrate plasma | Average % = 94 | Range 80 - 102 % |
Sample type EDTA Plasma | Average % = 105 | Range 100 - 113 % |
Sample type Heparin Plasma | Average % = 103 | Range 93 - 117 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human IL-1ra ELISA Kit (ab211650) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1ra protein in milk, plasma, saliva, serum, urine, cell culture extracts, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-1ra with 4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-1 receptor antagonist (IL-1ra, also known as IL-1F3) is a member of the IL-1 cytokine family. IL-1ra inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. However, IL-1ra has no interleukin-1 like activity. It binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
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12 product images
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Human IL-1RA standard curve comparison data.
Standard curve comparison between human IL-1RA SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Example of human IL-1Ra standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Example of human IL-1Ra standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Interpolated concentrations of native IL-1Ra in human serum and plasma samples.
The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (EDTA) 100%, plasma (heparin) 100%; and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 276 pg/mL in serum, 239 pg/mL in plasma (EDTA) and 233 pg/mL in plasma (heparin) and 170 pg/mL in plasma (citrate).
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 277 pg/mL with a range of 130 – 598 pg/mL.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Interpolated concentrations of native IL-1Ra in human saliva, milk and urine samples.
The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 1%, milk 15%, and urine 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 207 ng/mL in saliva, 1,121 pg/mL in milk and 4,271 pg/mL in urine.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Interpolated concentrations of native IL-1Ra in unstimulated and PHA/PMA stimulated human Peripherial Blood Monocyte (PBMC) Cell samples.
The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 2% and stimulated 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 3360 pg/mL in unstimulated, 11,912 pg/mL in stimulated and undetectable in media.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Sandwich ELISA - Human IL-1RA knockout A-431 and THP-1 cells.
Human IL-1RA concentration was interpolated from the IL-1RA standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-1ra ELISA Kit (ab211650). Wild-type A-431, IL1RN knockout A-431 (Human IL1RN (IL1 Receptor Antagonist) knockout A-431 cell line ab273379) and THP-1 cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Example of human IL-1Ra standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Interpolated concentration of native IL-1Ra was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Example of human IL-1Ra standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-1ra ELISA Kit (ab211650)
Interpolated concentration of native IL-1Ra was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 7.5 µg/mL A431 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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