AB211650

Human IL-1ra ELISA Kit

KO Validated
Recombinant
SimpleStep
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(7 Publications)

Human IL-1ra ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-1ra with a sensitivity of 1.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity- Cited in over 5 citations

View Alternative Names

IL1F3, IL1RA, IL1RN, Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor

13 Images

ELISA - Human IL-1ra ELISA Kit (AB211650)

ELISA

Supplier Data

ELISA - Human IL-1ra ELISA Kit (AB211650)

sELISA

Supplier Data

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 277 pg/mL with a range of 130 – 598 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Example of human IL-1Ra standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Interpolated concentrations of native IL-1Ra in human saliva, milk and urine samples.

The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 1%, milk 15%, and urine 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 207 ng/mL in saliva, 1,121 pg/mL in milk and 4,271 pg/mL in urine.

sELISA

Unknown

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Human IL-1RA standard curve comparison data.

Standard curve comparison between human IL-1RA SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

sELISA

Unknown

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Example of human IL-1Ra standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Interpolated concentrations of native IL-1Ra in unstimulated and PHA/PMA stimulated human Peripherial Blood Monocyte (PBMC) Cell samples.

The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated 2% and stimulated 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 3360 pg/mL in unstimulated, 11,912 pg/mL in stimulated and undetectable in media.

sELISA

Supplier Data

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Interpolated concentrations of native IL-1Ra in human serum and plasma samples.

The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (EDTA) 100%, plasma (heparin) 100%; and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 276 pg/mL in serum, 239 pg/mL in plasma (EDTA) and 233 pg/mL in plasma (heparin) and 170 pg/mL in plasma (citrate).

sELISA

Lab

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1RA knockout A-431 and THP-1 cells.

Human IL-1RA concentration was interpolated from the IL-1RA standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-1ra ELISA Kit (ab211650). Wild-type A-431, IL1RN knockout A-431 (ab273379) and THP-1 cells were assessed in duplicate (n=2). Data are represented as the mean and error bars represent standard deviation.

sELISA

Lab

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Example of human IL-1Ra standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Interpolated concentration of native IL-1Ra was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Interpolated concentration of native IL-1Ra was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 7.5 µg/mL A431 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Example of human IL-1Ra standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Milk, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.5 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-1ra ELISA Kit ab211650 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-1ra in cell culture extracts, cell culture supernatant, milk, plasma, saliva, serum, tissue extracts, urine. This SimpleStep sensitivity is 1.5 pg/mL.

How the assay works

Human IL-1ra SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-1ra ELISA Kit ab211650 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human IL-1ra ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-1RA antibody [EPR18693-54] - BSA and Azide free (Capture) ab242974
- Anti-IL-1RA antibody [EPR18693-60] - BSA and Azide free (Detector) ab242974

Interleukin-1 receptor antagonist (IL-1ra, also known as IL-1F3) is a member of the IL-1 cytokine family. IL-1ra inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. However, IL-1ra has no interleukin-1 like activity. It binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "EDTA Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "EDTA Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 99 %", "average": "= 98" }, { "sample": "Milk", "range": "98 - 110 %", "average": "= 104" }, { "sample": "Serum", "range": "82 - 119 %", "average": "= 106" }, { "sample": "Urine", "range": "100 - 106 %", "average": "= 102" }, { "sample": "Saliva", "range": "96 - 112 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "80 - 102 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "100 - 113 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "93 - 117 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.

See full target information IL1RN

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 16: PubMed39771498

2025

The Intra-Articular Delivery of a Low-Dose Adeno-Associated Virus-IL-1 Receptor Antagonist Vector Alleviates the Progress of Arthritis in an Osteoarthritis Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Luo,Hao Jiang,Qingwei Li,Shiping Yang,Xuemei Yu,Xiongliang Xu,Qing Xie,Xiao Ke,Qiang Zheng

Physiological reports 12:e16020 PubMed38658362

2024

Effect of epicatechin consumption on the inflammatory pathway and mitochondria morphology in PBMC from a R350P desminopathy patient: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Germán Tapia-Curimil,Mauricio Castro-Sepulveda,Hermann Zbinden-Foncea

iScience 26:106873 PubMed37250788

2023

induces trained immunity in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Miquel de Homdedeu,Lidia Sanchez-Moral,Concepció Violán,Neus Ràfols,Dan Ouchi,Berta Martín,Miguel A Peinado,Alhelí Rodríguez-Cortés,Marta Arch-Sisquella,Daniel Perez-Zsolt,Jordana Muñoz-Basagoiti,Nuria Izquierdo-Useros,Betlem Salvador,Joan Matllo,Sergi López-Serrano,Joaquim Segalés,Cristina Vilaplana,Pere Torán-Monserrat,Rosa Morros,Ramon Monfà,Maria-Rosa Sarrias,Pere-Joan Cardona

Cytokine 153:155868 PubMed35358903

2022

Clinical monitored in subjects metabolically healthy and unhealthy before and during a SARS-CoV-2 infection- A cross-sectional study in Mexican population.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Treviño,Steffany Cortezano-Esteban,Hugo Hernández-Fragoso,Alfonso Díaz,Rubén Vázquez-Roque,Victor Enrique Sarmiento-Ortega,Diana Moroni-González,Rosana Pelayo,Eduardo Brambila

Journal of neuroinflammation 18:189 PubMed34465372

2021

Interleukin-1 blockade attenuates white matter inflammation and oligodendrocyte loss after progressive systemic lipopolysaccharide exposure in near-term fetal sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Sharmony B Kelly,Vanesa Stojanovska,Valerie A Zahra,Alison Moxham,Suzanne L Miller,Timothy J M Moss,Stuart B Hooper,Marcel F Nold,Claudia A Nold-Petry,Justin M Dean,Laura Bennet,Graeme R Polglase,Alistair J Gunn,Robert Galinsky

Rheumatology (Oxford, England) : PubMed32259833

2020

New variant in the IL1RN-gene (DIRA) associated with late-onset, CRMO-like presentation.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin B Kuemmerle-Deschner,Tatjana Welzel,Konstanze Hoertnagel,Ilias Tsiflikas,Anton Hospach,Xiao Liu,Susanne Schlipf,Sandra Hansmann,Samuel D Samba,Andreas Griesinger,Susanne M Benseler,Alexander N Weber

Cellular immunology 327:77-82 PubMed29478949

2018

Interleukin-1β, interleukin-1 receptor antagonist, interleukin-6, interleukin-10, and tumor necrosis factor-α levels in CSF and serum in relation to the clinical diversity of Parkinson's disease.

Applications

ELISA

Species

Human

M N Karpenko,A A Vasilishina,E A Gromova,Z M Muruzheva,I V Miliukhina,A Bernadotte

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-1ra ELISA Kit

ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Sandwich ELISA - Human IL-1ra ELISA Kit (AB211650)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (7)

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Product promise