Human IL-1ra ELISA Kit (ab211650)

Human IL-1ra ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-1ra in Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Milk, Serum samples.

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Alternative names=Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, IL1RN, IL1F3, IL1RA

Detection method: Colorimetric
Sample types: Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Milk, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 7.81 - 500 pg/mL
Assay time: 1h 30m
Sensitivity: = 1.5 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Images

Target data

See full target information IL1RN

Function

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

What's included?

1 x 384 Tests

Components

10X Human IL-1Ra Capture Antibody

1 x 600 µL

10X Human IL-1Ra Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

50X Cell Extraction Enhancer Solution (ab193971)

6 x 1 mL

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent 4BI

1 x 6 mL

Human IL-1Ra Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

1 x 50 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Recommended products

Alternative names=Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, IL1RN, IL1F3, IL1RA

Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Milk, Serum
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 7.81 - 500 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 1.5 pg/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample EDTA Plasma	n 5	mean -	SD -	C.V. 3.5

Inter assay

Sample	n	mean	SD	C.V.
Sample EDTA Plasma	n 3	mean -	SD -	C.V. 4.3

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 98	Range 97 - 99 %
Sample type Milk	Average % = 104	Range 98 - 110 %
Sample type Serum	Average % = 106	Range 82 - 119 %
Sample type Urine	Average % = 102	Range 100 - 106 %
Sample type Saliva	Average % = 102	Range 96 - 112 %
Sample type Citrate plasma	Average % = 94	Range 80 - 102 %
Sample type EDTA Plasma	Average % = 105	Range 100 - 113 %
Sample type Heparin Plasma	Average % = 103	Range 93 - 117 %

Storage

Shipped at conditions: Blue Ice
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human IL-1ra ELISA Kit (ab211650) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-1ra protein in milk, plasma, saliva, serum, urine, cell culture extracts, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-1ra with 4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-1 receptor antagonist (IL-1ra, also known as IL-1F3) is a member of the IL-1 cytokine family. IL-1ra inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. However, IL-1ra has no interleukin-1 like activity. It binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Visit the general protocols
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