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Human IL-1ra ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IL-1ra with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

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Images

Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (AB229435), expandable thumbnail
  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (AB229435), expandable thumbnail
  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (AB229435), expandable thumbnail
  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (AB229435), expandable thumbnail
  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (AB229435), expandable thumbnail

Key facts

Detection method
Fluorescent
Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
3.9 - 2000 pg/mL
Assay time
1h 30m
Sensitivity
= 1.4 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.

Alternative names

What's included?

1 x 96 Tests
Components
100X Stoplight Red Substrate
1 x 120 µL
10X Human IL-1Ra Capture Antibody
1 x 600 µL
10X Human IL-1Ra Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
500X Hydrogen Peroxide (H2O2, 3%)
1 x 50 µL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent 4BI
1 x 6 mL
Human IL-1Ra Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated Black 96-Well Microplate
1 x 1 Unit
Stoplight Red Substrate Buffer
1 x 12 mL

Recommended products

Human IL-1ra ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IL-1ra with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

Key facts

Detection method
Fluorescent
Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
3.9 - 2000 pg/mL
Assay time
1h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 1.4 pg/mL

Precision

Intra assay

Sample
Plasma
n
5
C.V.
3.5

Inter assay

Sample
Plasma
n
3
C.V.
4.3

Recovery

Sample specific recovery

Sample type
Milk
Average %
= 104
Range
98 - 110 %
Sample type
Serum
Average %
= 106
Range
82 - 119 %
Sample type
EDTA Plasma
Average %
= 105
Range
100 - 113 %
Sample type
Saliva
Average %
= 102
Range
96 - 112 %
Sample type
Urine
Average %
= 102
Range
100 - 106 %
Sample type
Citrate plasma
Average %
= 94
Range
80 - 102 %
Sample type
Cell culture media
Average %
= 98
Range
97 - 99 %
Sample type
Plasma
Average %
= 103
Range
93 - 117 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

IL-1Ra in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1Ra protein in human serum, plasma, cell culture supernatants, milk, saliva, and urine.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Interleukin-1 receptor antagonist (IL-1ra, also known as IL-1F3) is a member of the IL-1 cytokine family. IL-1ra inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. However, IL-1ra has no interleukin-1 like activity. It binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435), expandable thumbnail

    Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435)

    Example of human IL-1Ra standard curve in Sample Diluent NS.

    The IL-1Ra standard curve was prepared as described in Section 10.

  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435), expandable thumbnail

    Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435)

    Interpolated concentrations of native IL-1Ra in human serum and plasma samples.

    The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (EDTA) 100%, plasma (heparin) 100%; and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 276 pg/mL in serum, 239 pg/mL in plasma (EDTA) and 233 pg/mL in plasma (heparin) and 170 pg/mL in plasma (citrate).

  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435), expandable thumbnail

    Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435)

    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 277 pg/mL with a range of 130 – 598 pg/mL.

  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435), expandable thumbnail

    Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435)

    Interpolated concentrations of native IL-1Ra in human saliva, milk and urine samples.

    The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 1%, milk 15%, and urine 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 207 ng/mL in saliva, 1,121 pg/mL in milk and 4,271 pg/mL in urine.

  • Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435), expandable thumbnail

    Sandwich ELISA - Human IL-1ra ELISA Kit, Fluorescent (ab229435)

    Interpolated concentrations of native IL-1Ra in unstimulated and PHA/PMA stimulated human Peripherial Blood Monocyte (PBMC) Cell samples.

    The concentrations of IL-1Ra were measured in duplicates, interpolated from the IL-1Ra standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 2% and stimulated 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-1Ra concentration was determined to be 3360 pg/mL in unstimulated, 11,912 pg/mL in stimulated and undetectable in media.

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Product protocols

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