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AB270883

Human IL-2 ELISA Kit

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(5 Publications)

Human IL-2 ELISA Kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-2 (IL-2) with a sensitivity of 32.1 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric Sandwich ELISA - 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Broad sample compatibility – suitable for cell culture media, cell culture supernatant, serum and plasma
- Cited in over 10 publications

View Alternative Names

Interleukin-2, IL-2, T-cell growth factor, TCGF, IL2

13 Images
ELISA - Human IL-2 ELISA Kit (AB270883)
  • ELISA

Supplier Data

ELISA - Human IL-2 ELISA Kit (AB270883)
Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Lab

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Example of human IL-2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-2 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent 50BP.

50% pooled serum samples from eight healthy donors was measured in duplicate. All values were below the detectable range of the assay.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Example of human IL-2 standard curve in Sample Diluent NS.

The IL-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Example of human IL-2 standard curve in Sample Diluent 50BP. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Example of human IL-2 standard curve in Sample Diluent 50BP. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Example of human IL-2 standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Assay sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Human IL-2 standard curve comparison.

Standard Curve comparison between the original Human IL-2 SimpleStep ELISA kit (ab174444) and current Human IL-2 SimpleStep ELISA kit (ab270883).

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Linearity of dilution.

Native IL-2 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in NS.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Linearity of dilution.

Recombinant IL-2 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Lab

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Example of human IL-2 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-2 ELISA Kit (AB270883)

Interpolated concentrations of native IL-2 in human stimulated PBMCs.

The concentrations of IL-2 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMCs stimulated with 1.5% PHAM for 48 hours (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 5,330 pg/mL in stimulated PBMCs.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 32.1 pg/mL

Range

39 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-2 ELISA Kit ab270883 is a rapid single-wash 90-min ELISA kit to measure Human IL-2 in cell culture media, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 32.1 pg/mL.

How the assay works
Human IL-2 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant human IL-2 protein in stimulated serum, plasmas, and cell culture supernatant. Urine, milk, saliva, and cell and tissue extract samples have not been tested with this kit. Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant human IL-2R alpha was prepared at 5,000 pg/mL and tested for interference. No interference with was observed.

Human IL-2 ELISA Kit ab270883 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IL-2 ELISA Kit ab270883
Human IL-2 ELISA Kit ab270883 has been used to study the role of long noncoding RNA in immunotherapy resistance(1)and exosomal effects of HLA transfer on T lymphocytes in Celiac Disease(2).

References:
(1)Hui-Yan Luo et al. 2023, PMID: 37712124,
(2)Valerie Brid O'Leary et al. 2022, PMID: 35682780

Save your precious samples
Our 384-well Human IL-2 ELISA Kit only requires a maxmum of 12.5µL

Related and recommended products
Human IL-2 ELISA Kit ab270883 is commonly used to measure human interleukin 2 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit ab181421
- Human IL-6 ELISA kit ab178013
- Human IFN gamma ELISA kit ab174443

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 120 %", "average": "= 108" }, { "sample": "Serum", "range": "83 - 88 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "86 - 93 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "80 - 85 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "84 - 91 %", "average": "= 87" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed : 6438535). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) (PubMed : 16293754, PubMed : 16477002). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3 (PubMed : 7973659). In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed : 8580378). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways (PubMed : 25142963). Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well (PubMed : 6608729). Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production (PubMed : 6438535). Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
See full target information IL2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:3118-3157 PubMed39072171

2024

Kombo knife combined with sorafenib in liver cancer treatment: Efficacy and safety under immune function influence.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cao,Pei-Pei Li,Bing-Li Qiao,Quan-Wang Li

Journal of translational medicine 22:679 PubMed39054481

2024

Humanization of the antigen-recognition domain does not impinge on the antigen-binding, cytokine secretion, and antitumor reactivity of humanized nanobody-based CD19-redirected CAR-T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pooria Safarzadeh Kozani,Pouya Safarzadeh Kozani,Fatemeh Rahbarizadeh

Journal of immunology (Baltimore, Md. : 1950) 212:917-927 PubMed38214607

2024

Efficacy against Lung Cancer Is Augmented by Combining Aberrantly N-Glycosylated T Cells with a Chimeric Antigen Receptor Targeting Fragile X Mental Retardation 1 Neighbor.

Applications

Unspecified application

Species

Unspecified reactive species

Toshihiko Toyofuku,Takako Ishikawa,Satoshi Nojima,Atsushi Kumanogoh

Clinical and translational medicine 13:e1410 PubMed37712124

2023

Long noncoding RNA Regulating ImMune Escape regulates mixed lineage leukaemia protein-1-H3K4me3-mediated immune escape in oesophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Liu,Wei-Yi Zhou,Xiao-Jing Luo,Yan-Xing Chen,Chau-Wei Wong,Ze-Xian Liu,Jia- Bo Zheng,Hai- Yu Mo,Jun-Quan Chen,Jia-Jun Li,Ming Zhong,Yu-Hong Xu,Qi-Hua Zhang,Heng-Ying Pu,Qi-Nian Wu,Ying Jin,Zi-Xian Wang,Rui-Hua Xu,Hui-Yan Luo

International journal of molecular sciences 23: PubMed35682780

2022

Enhanced Extracellular Transfer of HLA-DQ Activates CD3 Lymphocytes towards Compromised Treg Induction in Celiac Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hudec,Iva Juříčková,Kamila Riegerová,Saak V Ovsepian,Marie Černá,Valerie Bríd O'Leary
View all publications
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