Human IL-2 ELISA Kit, Fluorescent

Product details

IL-2 (Interleukin-2) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 (Interleukin-2) protein in human cell culture supernatant, plasma and serum samples.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

IL-2, also known as T cell growth factor (TCGF), is a glycosylated alpha-helical polypeptide, synthesized as a 153 amino acid (aa) precursor with a 20 aa signal peptide and a 133 aa mature chain. It is secreted by activated CD4⁺ and CD8⁺ T cells, neurons, microglia and hematopoietic stem cells in response to antigenic or mitogenic stimulation. IL-2 is required for T-cell proliferation, Natural Killer cells (NK) cytolytic activity, differentiation of regulatory T cells, modulation of T helper (Th) cell differentiation and activation-induced cell death. In particular, IL-2 modulates the expression of receptors for other cytokines and transcription factors, therefore regulating cytokine cascades that correlate with each of the Th differentiation states.

Complete deficiency of IL-2 has been implicated in severe combined immunodeficiency, whereas reduction of the IL-2 correlates with reduced function of CD4⁺CD25⁺ regulatory T cells and destabilization of immune homeostasis leading to autoimmune disease. Increased expression of IL-2 has also been implicated in inflammatory conditions such as inflammatory bowel disease and chronic liver diseases. IL-2 therefore is both a immune stimulator and immune suppressor cytokine which efficiently controls the immune system to deal with autoimmunity and adaptive immune response.