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AB46036

Human IL-2 Receptor ELISA Kit

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(2 Publications)

Human IL-2 Receptor ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-2 Receptor in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

1 Images
Sandwich ELISA - Human IL-2 Receptor ELISA Kit (AB46036)
  • sELISA

Unknown

Sandwich ELISA - Human IL-2 Receptor ELISA Kit (AB46036)

Representative Standard Curve using ab46036

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 32.5 pg/mL

Range

68.75 - 2200 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-2 Receptor Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 Receptor in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant Human IL-2 Receptor.

A monoclonal antibody specific for IL-2 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 Receptor present in the samples.

This kit will recognize both endogenous and recombinant Human IL-2 Receptor.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 6, "mean": "1594 pg/mL", "standardDeviation": "184", "coefficientOfVariability": "11.6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 6, "mean": "1528 pg/mL", "standardDeviation": "135", "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 6, "mean": "1705 pg/mL", "standardDeviation": "204", "coefficientOfVariability": "12" }, { "reproducibilityType": "Inter", "sample": "Sample 4", "replicates": 6, "mean": "1124 pg/mL", "standardDeviation": "171", "coefficientOfVariability": "15.2" }, { "reproducibilityType": "Inter", "sample": "Sample 5", "replicates": 6, "mean": "2305 pg/mL", "standardDeviation": "165", "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Inter", "sample": "Sample 6", "replicates": 6, "mean": "1824 pg/mL", "standardDeviation": "126", "coefficientOfVariability": "69" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 122 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46036-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46036-CMP04" }, { "size":"2 x 1 Vial", "name":"IL-2 Receptor Control", "number":"AB46036-CMP11" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46036-CMP06" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46036-CMP07" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46036-CMP05" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-IL-2 Receptor", "number":"AB46036-CMP01" }, { "size":"1 x 1 Unit", "name":"IL-2 Receptor Microplate (12 x 8 well strips)", "number":"AB46036-CMP08" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46036-CMP02" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46036-CMP03" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46036-CMP09" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46036-CMP10" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46036-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46036-CMP04", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-2 Receptor Control", "number":"AB46036-CMP11", "productcode":"" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-IL-2 Receptor", "number":"AB46036-CMP01", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46036-CMP03", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46036-CMP02", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46036-CMP05", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-2 Receptor Microplate (12 x 8 well strips)", "number":"AB46036-CMP08", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46036-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46036-CMP07", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46036-CMP09", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46036-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
See full target information IL2RA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cancer research and clinical oncology 145:411-427 PubMed30483898

2018

Effects of VEGFR1 hematopoietic progenitor cells on pre-metastatic niche formation and in vivo metastasis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Du Meng,Min Meng,Anqi Luo,Xin Jing,Guanying Wang,Shangke Huang,Minna Luo,Shan Shao,Xinhan Zhao,Rui Liu

BMC complementary and alternative medicine 16:300 PubMed27550057

2016

Evaluation of the immunomodulatory effects of a South African commercial traditional immune booster in human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mlungisi Ngcobo,Nceba Gqaleni
View all publications
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websiteProtocolBooklet
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